297 / 658
Conclusion
A robust method with simple and easy sample preparation
was developed for forensic toxicology laboratories. The
data window and total run time make this method ame-
nable to multiplexing with the Thermo Scientific
LQC
MQC
HQC
%Bias
%CV
%Bias
%CV
%Bias
%CV
Morphine
-9.42
8.72
2.92
3.29
4.50
2.24
Oxymorphone
12.2
3.45
7.50
2.35
0.00
4.16
Hydromorphone
-1.92
9.79
0.0833
6.63
-4.17
4.93
Codeine
-7.92
17.2
1.50
3.21
3.25
3.46
Oxycodone
-8.08
8.99
8.17
2.24
5.17
2.44
Hydrocodone
-2.42
8.84
7.25
3.60
5.58
4.07
Compound
Lot 1
Lot 2
Lot 3
Lot 4
Lot 5
Lot 6
Lot 7
Morphine
92.0
98.9
98.9
91.6
96.4
103
94.2
Oxymorphone
105
109
110
116
115
107
113
Hydromorphone
117
93.5
81.5
89.5
101
98.9
92.7
Codeine
113
113
104
98.9
112
108
103
Oxycodone
85.8
97.8
100
103
101
84.4
89.8
Hydrocodone
103
95.6
99.6
99.3
86.5
119
118
Table 1. Intra-assay quality control %Bias and %CV
Table 2. Percent recovery of six synthetic opioids in seven lots of human urine
1.8
1.9
2.0
2.1
2.2
2.3
2.4
2.5
2.6
2.7
2.8
2.9
3.0
3.1
Time (min)
0
50
100
Relative Abundance
Morphine
Oxymorphone
Hydromorphone
Codeine
Oxycodone
Hydrocodone
Opiates app note: Figure 2
Figure 2. Representative chromatogram of six opiates in urine at LOQ of 10 ng/mL
% Recovery
Transcend system. Multiplexing with the Transcend™
LX-2 LC system would result in a run time of 2.5 minutes
per sample. With an LX-4 LC system, the run time could
be further reduced to 1.53 minutes per sample.