293 / 658
Conclusion
A robust method with simple and easy sample preparation
was developed and validated for forensic toxicology
laboratories. The data window and total run time make
Table 1. Inter-assay quality control statistics for validation runs
Methadone EDDP
Meperidine Normeperidine Propoxyphene Norpropoxyphene
LQC
4.16/4.71
2.67/6.04
0.493/5.93
7.04/9.17
5.45/3.42
3.28/8.64
MQC
7.32/2.47
-5.72/4.48
3.55/5.31
-0.747/7.61
4.36/5.28
0.933/3.88
HQC
10.9/2.69 -0.587/2.28
5.67/4.13
3.93/5.92
1.81/5.96
-3.77/8.18
%Bias/%CV
Table 2. Percent recovery of six synthetic opioids in eight lots of human urine
Compound
Lot A
Lot B
Lot C
Lot D
Lot E
Lot F
Lot G
Lot H
Methadone
105
92.5
97.3
103
98.5
98.5
99.3
98.4
EDDP
94.9
97.1
95.1
96.0
105
92.4
94.9
98.2
Meperidine
104
97.6
105
98.4
110
104
98.7
102
Normeperidine
117
115
111
94.7
105
111
108
118
Propoxyphene
98.7
97.3
96.4
99.8
98.7
89.1
95.5
102
Norpropoxyphene
97.8
92.2
87.3
101
94.5
96.4
97.1
104
% Recovery
Figure 2. SRM chromatogram of six synthetic opioids and metabolites in urine at 20 ng/mL
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
3.0
3.2
3.4
3.6
3.8
4.0
4.2
4.4
Time (min)
Meperidine
Normeperidine
Norpropoxyphene
Propoxyphene
EDDP
Methadone
Figure 2: Reconstructed SRM chromatogram of six synthetic opioids and metabolites in urine
this method amenable to multiplexing with the Thermo
Scientific Transcend LX-2 LC system. Multiplexing with
the Transcend™ LX-2 LC system would result in a run
time of 2.5 minutes per sample.