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Conclusion
TraceFinder 1.1 software was effectively used to perform
routine analysis of the synthetic opiates in urine. The
software enabled easy method setup, batch creation and
submission, and real time monitoring. The data review
functionality was useful for quick review and verification
of the data. The generated reports had all the necessary
information for record keeping for forensic laboratories.
Results and Discussion
The method was linear from 20 to 5000 ng/mL for five of
the six compounds. Normeperidine was linear from 2 to
1000 ng/mL. Standard accuracy ranged between 87.3%
and 115%. Matrix effects were investigated by analyzing
QCs prepared from six different lots of blank human
urine. All samples showed recoveries within 20% at
50 ng/mL. The assay performance is summarized in
Table 1. Figure 10 shows the SRM chromatograms of all
six synthetic opiates at the limit of quantitation (LOQ).
Table 1. Assay performance for six synthetic opiates in urine
% Recovery
Lot 1
Lot 3
Lot 4
Lot 5
Lot 6
Lot 7
Lot 8
Lot 9
R
2
Linear Range
LOQ
Methadone
105.0% 92.5% 97.3% 103.0% 98.5% 98.5% 99.3% 98.4% 0.9945 20-5000 ng/mL 20
EDDP
94.9% 97.1% 95.1% 96.0% 105.0% 92.4% 94.9% 98.2% 0.9951 20-5000 ng/mL 20
Meperidine
104.0% 97.6% 105.0% 98.4% 110.0% 104.0% 98.7% 102.0% 0.9935 20-5000 ng/mL 20
Normeperidine
117.0% 115.0% 111.0% 94.7% 105.0% 111.0% 108.0% 118.0% 0.9998
2-1000 ng/mL
2
Propoxyphene
98.7% 97.3% 96.4% 99.8% 98.7% 89.1% 95.5% 102.0% 0.9994 20-5000 ng/mL 20
Norpropoxyphene-
dehydrate
97.8% 92.2% 87.3% 101.0% 94.5% 96.4% 97.1% 104.0% 0.9989 20-5000 ng/mL 20