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5
Analyte
Calibration Range
(ng/mL)
Intercept
Slope
R
2
Carbamazepine
1.62 – 15.1
-0.068
0.610
0.998
Oxcarbazepine
0.14 – 3.64
-0.054
1.585
0.998
Carbamazepine diol
0.16 – 11.5
0.000
0.115
0.996
Carbamazepine-10,11-epoxide
0.16 – 11.0
-0.056
7.099
0.995
10-hydroxycarbamazepine
3.83 – 48.3
0.072
0.347
0.999
Felbamate
14.3 – 130
-0.119
0.484
0.995
Lacosamide
0.78 – 11.4
-0.064
1.145
0.996
Levetiracetam
4.90 – 84.0
-0.451
0.954
0.995
Rufinamide
3.98 – 35.3
-0.449
1.026
0.996
Theophylline
6.11 – 24.6
0.117
0.198
0.991
Phenytoin
3.49 – 23.3
-2.185
0.667
0.996
Primidone
3.07 – 18.3
2.570
1.375
0.997
PEMA
1.07 – 12.7
-1.964
6.885
0.996
Stiripentol
2.62 – 27.3
-2.298
1.922
0.994
Phenobarbital
7.38 – 60.1
-0.027
0.083
0.998
Valproic Acid
31.6 – 125
-0.041
0.011
0.998
Zonisamide
3.97 – 50.4
-0.157
0.286
0.999
Table 6. Calibration range, intercept, slope, and correlation factor (R
2
)
Representative calibration curves for carbamazepine and phenobarbital are shown in Figure 1.
Representative chromatograms at the limit of quantitation (LOQ) for each analyte, including the
internal standards, are shown in Figures 2, 3, and 4.
Figure 1. Calibration curves for (a) carbamazepine and (b)
phenobarbital
Figure 2. Representative chromatograms of each analyte at the LOQ and corresponding IS
for Group 1
0
5
10
15
ng/ml
0
2
4
6
8
10
(a) Carbamazepine
0
10
20
30
40
50
60
ng/ml
0
1
2
3
4
5
(b) Phenobarbital
0.5
1.0
1.5
2.0
2.5
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
Relative Abundance
0
20
40
60
80
100
0
20
40
60
80
100
RT: 1.23
RT: 1.39
RT: 1.82
RT: 1.79
RT: 1.79
RT: 1.38
RT: 1.80
RT: 1.79
RT: 1.79
2.0
2.5
3.0
3.5
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
RT: 2.19
RT: 2.43
RT: 2.40
RT: 2.43
RT: 3.43
2.0
2.5
3.0
3.5
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
RT: 2.38
RT: 2.37
RT: 2.38
RT: 2.88
RT: 3.42
IS3
IS2
IS4
IS3
IS1
Levetiracetam
Theophylline
Rufinamide
Felbamate
Lacosamide
Carbamazepine
Diol
Carbamazepine
10,11-epoxide
Oxcarbazepine
10-hydroxy
Carbamazepine
Carbamazepine
IS7
IS8
IS6
IS5
IS6
RT: 1.22