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Quantitative and Semi-Quantitative Determination of PPCPs and Their By-products in Wastewater Treatment Plants Samples Using UHPLC-Orbitrap MS
and Data Mining Technologies
Conclusion
Quantitative results of PPCPs were obtained using HPLC-Orbitrap MS.
Semi-quantitative results, seasonal trends and effect of treatment temperature on
PPCP by-products were obtained using TraceFinder and SIEVE software.
Efforts to obtain analytical standards to complete the studies are on-going.
References
1.
“The Determination of Emerging Organic Pollutants in Environmental Matrices by
Liquid Chromatography-Tandem Mass Spectrometry (LC-
MS/MS)”, Ontario
Ministry of the Environment method E3454. Available via
laboratoryservicesbranch@ontario.ca.2.
“Monitoring
the photochemical degradation of triclosan in wastewater by UV light
and sunlight using solid-phase microextraction
”. Sanchez
-Prado, L., Llompart,
M., Lores, M., García-Jares, C., Bayona, J. M., & Cela, R. (2006). Chemosphere,
65(8), pp. 1338-1347.
Acknowledgements
We would like to thank Dr. Vince Pileggi for providing permeate samples; as well as
Ms. Renee Luniewski for providing valuable suggestions to make this work complete.
(anticonvulsant drug) will be
uring wastewater treatment
nd are the two most studied
res and seasonal changes
sis. As shown in Figure 2,
55
°
C (brown, winter) were
reen, winter) and standards
temperatures exerted more
he literature
2
, five
chlorophenol (Cl-Ph),
(Cl-TCS)). Semi-
(TCS-D3), Cl-Ph, Cl-TCS
f Cl-Ph were minimum
012
55
°
C, Jan 2013
CS by-products found
In comparison with other PPCPs studied, CBZ had the most by-products identified (16)
in this work. The unequivocal identification for CBZ by-products thus became a
challenge as many of these compounds had the same chemical formula and therefore,
the same monoisotopic mass measured by the Orbitrap MS. Without available
reference standards, the chromatographic peak was assigned to the most probable
structure with the most dominated population in the literature. Semi-quantitative
concentrations of CBZ, deuterium labelled CBZ (CBZ-D10), and the three by-products
found are shown in Figure 4.
Figure 4. Relative concentration of CBZ and the three CBZ by-products found
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