297 / 302
7
References
1. Analysis of emerging persistent organic pollutants using
GC-MS/MS; Kalachova
et al
. SETAC, Berlin 2012.
2. Ziegenhals, K.; Hubschmann, H.J. Fast-GC/HRMS to
quantify the EU priority PAH.
J. Sep. Sci.
2008
,
31,
1779 – 1786.
3. Thermo Scientific Application Brief AB52998:
Introducing AutoSRM: MRM Simplicity for High
Performance Results; Cole J.
4. REGULATION (EC) No 2002/657 on analytical
performance criteria.
5. Pesticides Method Reference, 2
nd
ed. 2011, Thermo
Fisher Scientific, Austin, TX, USA, P/N 120390.
Conclusions
• The TSQ 8000 GC-MS/MS enabled simple method
development, validation, and management using a
combination of integrated software tools.
• The new method increased productivity in the
laboratory by combining three separate methods into
one, from three injections down to one injection.
• Quantitative performance of the system and
methodology was excellent with a good level of
linearity, excellent sensitivity, and high precision in a
variety of environmental sample types.
Parent Mass
(Da)
Product Mass
(Da)
Collision Energy
(V)
RT
(min)
Start Time
*
(min)
Stop Time
*
(min)
Name
427.77
357.80
25
13.20
12.68
13.68 2,2',3,3',4,4',5,5'-Octachlorobiphenyl (BZ #194)
429.76
357.80
25
13.20
12.68
13.68
2,2',3,3',4,4',5,5'-Octachlorobiphenyl (BZ #194)
Confirming 1
391.81
321.84
25
11.70
11.16
12.16 2,2',3,4,4',5,5'-Heptachlorobiphenyl (BZ #180)
393.81
323.84
25
11.70
11.16
12.16
2,2',3,4,4',5,5'-Heptachlorobiphenyl (BZ #180)
Confirming 1
357.84
287.88
25
10.20
9.70
10.70 2,2',4,4',5,5'-Hexachlorobiphenyl (BZ #153)
359.84
289.87
25
10.20
9.70
10.70
2,2',4,4',5,5'-Hexachlorobiphenyl (BZ #153)
Confirming 1
289.92
219.94
20
7.87
7.37
8.37 2,2',5,5'-Tetrachlorobiphenyl (BZ #52)
291.92
219.94
20
7.87
7.37
8.37 2,2',5,5'-Tetrachlorobiphenyl (BZ #52) Confirming 1
323.88
253.91
20
9.88
9.38
10.38 2,3',4,4',5-Pentachlorobiphenyl (BZ #118)
325.88
255.91
20
9.88
9.38
10.38 2,3',4,4',5-Pentachlorobiphenyl (BZ #118) Confirming 1
255.96
185.97
20
7.48
6.98
7.98 2,4,4'-Trichlorobiphenyl (BZ #28)
257.96
185.97
20
7.48
6.98
7.98 2,4,4'-Trichlorobiphenyl (BZ #28) Confirming 1
153.07
126.05
45
5.58
5.08
6.08 acenaphtene
153.07
151.07
40
5.58
5.08
6.08 acenaphtene Confirming 1
164.14
160.00
30
5.55
5.05
6.05 acenaphtene D10
164.14
162.00
20
5.55
5.05
6.05 acenaphtene D10 Confirming 1
152.06
102.03
30
5.43
4.93
5.93 acenaphtylene
152.06
126.05
20
5.43
4.93
5.93 acenaphtylene Confirming 1
276.08
272.08
60
16.70
16.20
17.20 benzo[ghi[perylene
276.08
274.08
40
16.70
16.20
17.20 benzo[ghi[perylene Confirming 1
216.89
180.91
8
6.48
5.98
6.98 BHC-alpha (benzene hexachloride)
218.89
182.91
8
6.48
5.98
6.98 BHC-alpha (benzene hexachloride) Confirming 1
240.17
212.00
30
11.40
10.94
11.94 chrysene D12
240.17
236.00
30
11.40
10.94
11.94 chrysene D12 Confirming 1
235.01
164.98
20
9.50
9.00
10.00 DDD-o,p'
237.01
164.98
20
9.50
9.00
10.00 DDD-o,p' Confirming 1
495.69
425.73
25
14.30
13.79
14.79 Decachlorobiphenyl (BZ #209)
497.69
427.73
25
14.30
13.79
14.79 Decachlorobiphenyl (BZ #209) Confirming 1
278.08
274.08
60
16.30
15.82
16.82 dibenzo[ah]anthracene
Addendum: SRM Transitions
*
Start and Stop Times are set automatically in timed-SRM mode of the TSQ 8000 by using a standard acquisition window of 60 s for all compounds