LC-MS Applications for Environmental Analysis

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Table 5. Quantitative results for ethylacetate and LOD determination.
ETHYLACETATE
ISTD: Acetone d6 , syringe 1 mL, Speed 2
SRM (m/z 65 →m/z 33)
RT (min.)
Syringe
Speed Average Height
Background
Subtracted
10.5 OFF 26000
15.55 2 130873 104873
Ethylacetate
SRM (m/z 89 →m/z 61)
RT (min)
Syringe
Speed Average Height
Background
Subtracted
Response/ISTD
Ratio
Concentration
(µg/m 3)
1.2 - 2.0 9 32602596 22190201
3.4 - 4.2 7 27961558 17549163
5.5 - 6.3 5 22186498 11774103 112.3 317.8
7.5 - 8.3 3 15989249 5576854 53.2 127.1
9.4 - 10.2 1 13090732 2678337 25.5 63.6
OFF 10412395 0 0.0 0 Std. Dev. = 218065
(3 x Std. Dev.) = 654194 6.24 16.2 LOD
RT: 0.00 - 16.16 SM: 7B
0 2 4 6 8 10 12 14
Time (min)
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
RelativeAbundance
1589ug/m3
Av.Ht.=32602596
635.7ug/m3
Av.Ht.=27961558
317.8ug/m3
Av.Ht.=22186498
127.1ug/m3
Av.Ht.=15989249
63.6ug/m3
Av.Ht.=13090732
Background
Av.Ht.=10412395
NL:3.43E7
TIC F:+cAPCI
sid=5.00 SRMms2
8.00
[60.500-61.500] MS
acetethyle courb
Calibration Curve
ETHYLACETATE y = 0.0062x 2 + 2.1287x
R 2 = 0.9993
0
50
100
150
200
250
300
350
0 20 40 60 80 100 120
Conc. ug/m
3
Ratio (m/z 89 →m/z 61)/ISTD
Figure 4: Calibration curve for ethylacetate.
Figure 3: Reconstructed ion trace for ethylacetate to produce the calibration
curve.