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Conclusion
Full scan in negative mode LC-MS acquisition on the
Exactive LC-MS system with Orbitrap technology is
suitable for quantification of PFAAs at low concentrations
ranging from 0.1 µg/kg to 50 µg/kg in complex matrix
extracts. No tuning or fragment determination is required
and there is less background with high-resolution, accurate
mass acquisition.
Reference
1. Bossi, R.; Riget, F.F.; Dietz, R.; Sonne, C.; Fauser, P.; Dam, M.;
Vorkamp, K.
Environmental Pollution
2005
,
136,
323-329.
0
1
2
3
4
5
6
7
Time (min)
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
RT:2.95
BP:362.9700
4.23
362.9704
6.37
362.9687
1.41
362.9699
RT:3.98
BP:412.9680
5.42
412.9666
5.79
412.9662
0.07
412.9653
3.71
412.9654
RT:4.76
BP:462.9638
RT:6.40
BP:462.9633
RT:5.39
BP:512.9619
RT:5.90
BP:562.9578
RT:6.33
BP:612.9547
RT:6.71
BP:662.9509
0
1
2
3
4
5
6
7
Time (min)
0
50
100
0
50
100
0
50
100
0
50
100
Relative Abundance
0
50
100
0
50
100
0
50
100
4.86
362.9683
6.19
362.96
2.95
362.9680
1.69
362.9678
7.90
412.9671
5.72
412.9665
5.27
412.9664
5.99
412.9672
3.98
412.9661
1.88
412.9679
6.72
462.9635
6.06
462.9648
0.77
512.9608
4.57
512.9587
0.50
662.9536
4.27
662.9530
0
1
2
3
4
5
6
7
Time (min)
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
RT:3.93
BP:412.9674
RT:4.66
BP:462.9639
RT:5.27
BP:512.9612
RT:5.75
BP:562.9581
RT:6.12
BP:612.9557
RT:6.42
BP:662.9503
PFHpA
PFOA
PFNA
PFDA
PFUnA
PFDoA
PFTrA
PFHpA
PFOA
PFNA
PFDA
PFUnA
PFDoA
PFTrA
PFOA
PFNA
PFDA
PFUnA
PFDoA
PFTrA
PFOA
Y=0.0106602*X R^2=0.9993 W:Equal
0
5
10
15
20
25
30
35
40
45
50
55
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
0.50
0.55
AreaRatio
µg/kg
Figure 5. Extracted chromatograms: (a) Blank, (b) Standard, (c) Arctic seal extract
Figure 6. Calibration curve for PFOA
a) Blank
b) Standard
c) Arctic Seal Extract