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Conclusion
The current EPA Method 1694 describes three different
LC methods for PPCPs from Groups 1, 2, and 4, which are
amenable to positive ESI-MS/MS. To simplify the method
and reduce total analysis time, a single 10-minute LC-MS/
MS method was developed on the EQuan system includ-
ing compounds from additional pharmaceutical classes not
included in the EPA method, such as beta-blockers and
benzodiazepines.
The EQuan system significantly reduced sample
preparation and analysis time while providing quantifica-
tion of PPCPs in water at low ng/L levels. Online sample
preparation of the water samples eliminated the need to
use two different offline SPE methods on 1 L of water. This
reduced the total analysis time from hours to minutes. The
sensitivity of the TSQ Vantage mass spectrometer, using
time-dependent SRMs to maximize detector duty cycle,
provided low- or sub-ng/L limits of quantitation for the
targeted PPCPs in water.
References
1. EPA Method 1694: Pharmaceuticals and personal care products in water,
soil, sediment, and biosolids by HPLC/MS/MS, December
2007
, EPA-
821-R-08-002.
2. Loftin, K.A.; Adams, C.D.; Meyer, M.T.; Surampalli, R. “Effects of Ionic
Strength, Temperature, and pH on Degradation of Selected Antibiotic”
J.
Environ. Qual.
,
2008
, 37, 378-386.