114 / 302
pH 2.9
pH 11.3
pH 6.6
0.0
0.5
1.0
1.5
2.0
2.5
Time (min)
RT: 1.84
AA: 141940
RT: 1.81
AA: 137918
RT: 1.84
AA: 138950
RT: 1.07
AA: 85007
RT: 1.84
AA: 56218
0.0
0.5
1.0
1.5
2.0
2.5
Time (min)
RT: 1.86
AA: 133742
RT: 1.88
AA: 140409
RT: 1.84
AA: 137257
RT: 1.82
AA: 134771
0.0
0.5
1.0
1.5
2.0
2.5
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
RT: 1.83
AA: 146721
RT: 1.83
AA: 135126
RT: 1.41
AA: 71260
RT: 1.84
AA: 68484
RT: 1.06
AA: 96122
RT: 1.87
AA: 42653
20%
15%
10%
5%
Figure 6. Chromatograms showing the %ACN effect on LC column retention for cotinine.
4.0
4.5
5.0
5.5
6.0
6.5
7
Time (min)
RT: 6.04
AA: 17975
RT: 6.13
AA: 37475
RT: 6.29
AA: 19715
RT: 6.14
AA: 43273
RT: 6.20
AA: 8392
RT: 6.19
AA: 17142
RT: 6.20
AA: 16556
RT: 6.31
AA: 71593
3.5
4.0
4.5
5.0
5.5
6.0
6
Time (min)
RT: 5.41
AA: 16000
RT: 5.47
AA: 12712
RT: 5.50
AA: 4994
RT: 5.62
AA: 76608
RT: 5.63
AA: 4510
RT: 5.84
AA: 37897
RT: 5.95
AA: 8263
2
3
4
5
Time (min)
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
0
50
100
RT: 3.18
AA: 17332
RT: 4.60
AA: 4059
RT: 4.73
AA: 2342
RT: 4.77
AA: 14267
RT: 4.75
AA: 10378
RT: 4.87
AA: 10805
RT: 4.96
AA: 10283
Cotinine
(100 ng/L)
Albuterol
(10 ng/L)
Cimetidine
(1 ng/L)
Atenolol
(10 ng/L)
Acetaminophen
(100 ng/L)
Dimethylxanthine
(100 ng/L)
Ranitidine
(1 ng/L)
Codeine
(10 ng/L)
Sulfadiazine
(100 ng/L)
Lincomycin
(1 ng/L)
Caffeine
(100 ng/L)
Ampicillin
(100 ng/L)
Cefotaxime
(100 ng/L)
Sulfathiazole
(100 ng/L)
Trimethoprim
(1 ng/L)
Minocycline
(100 ng/L)
Sulfamerazine
(10 ng/L)
Norfloxacin
(10 ng/L)
Ofloxacin
(1 ng/L)
Benzoylecgonine
(1 ng/L)
Oxytetracycline
(100 ng/L)
Figure 7 (a,b,c). Example chromatograms of the PPCPs in water at the ng/L level. The LLOQ for each compound is listed in parentheses.
a