17
Group 2b – Radar plots for HILIC stationary phases with high cation exchange
properties
Further information on the data derived from this characterization testing regime, and on the individual column
chemistries is provided in the Column Characterization Detail section starting on page 35.
Column Geometry
As a general recommendation, for HILIC standard operations select a 100 or 150 mm long column, packed with 3−5 µm
particles and with a 4.6 mm id, if using UV detection.
If HILIC is coupled to MS or CAD detection, use a smaller column, e.g. 100 × 2.1 mm or 50 × 2.1 mm, packed with
3−5 µm particles (the reduction in column length should be undertaken after having considered sample complexity and
detector selectivity). For higher separation efficiency and resolution we suggest either sub 2 µm fully porous particles or
solid core materials or longer columns.
The optimum flow rate is dependent on the column internal diameter and particle size. Some investigations carried out
into the kinetic performance of 100 × 4.6 mm HILIC columns highlighted that for the same particle size and particle type
the optimum flow rate also varies with the column chemistry.
Hypersil GOLD Silica
(Tb/Tp)
(2dG/3dG)
k U /2
(CX) /2
(OH)
(V/A)
(AX)
(CH2)
Accucore HILIC
(Tb/Tp)
(2dG/3dG)
k U /2
(CX) /2
(OH)
(V/A)
(AX)
(CH2)
Syncronis Silica
(Tb/Tp)
(2dG/3dG)
k U /2
(CX) /2
(OH)
(V/A)
(AX)
(CH2)
