6. Calibration Standards
6.1 PAHs
Acenaphthene – Ace (Sigma)
Acenaphthylene – Acy (Sigma)
Anthracene – Ant (Sigma)
Benz[a]anthracene – B(a)A (Sigma)
Benzo[a]pyrene - B(a)P (Sigma)
Benzo[b]fluoranthene – B(b)F (Sigma)
Benzo[g,h,i]perylene - B(g,h,i)P (Sigma)
Benzo[k]fluoranthene - B(k)F (Sigma)
Chrysene – Chr (Sigma)
Dibenz[a,h]anthracene - D(a,h)A (Sigma)
Fluoranthene – Flu (Sigma)
Fluorene - Fln (Sigma)
Indeno(1,2,3-cd)pyrene - I(1,2,3-c,d)P (Sigma)
Naphthalene - Naph (Sigma)
Phenanthrene - Phe (Sigma)
Pyrene – Pyr (Sigma)
6.2 Injection Standard
5-methylchrysene – 5-MChr (Dr. Ehrenstorfer)
6.3 Internal Standards
Anthracene-D10 – Ant-D10 (Sigma)
Benzo[a]pyrene-D12 – B(a)P-D12 (Sigma)
Benzo[ghi]perylene-D12 – B(g,h,i)P-D12 (LGC Standards)
Chrysene-D12 – Chr-D12 (Sigma)
6.4 Quality Control Materials
FAPAS, smoked fish (T0642)
Petroleum Crude oil (NIST Standard Reference
Material
®
, 1582)
7. Standards and Reagent Preparation
7.1
MeOH/KOH solution, weigh 120 g KOH, add
60 mL of water and dilute in 900 mL Methanol
7.2
MeOH/H
2
O mixture: 400 mL of Methanol plus
100 mL of Water
7.3
Stock solutions of 2 µg/mL of PAH standards in
toluene
7.4
Internal PAHs standard (IS) concentration: is
2 µg/mL (Benzo(g,h,i)perylene-d12, Anthracene-d10,
Chrysene-d12) in toluene and 200µg/mL
Benzo(a)pyrene-d12 in cyclohexane
7.5
Working standard solution mixture of 16 PAHs in
toluene (100 ng/mL)
7.6
Working internal standard mixture of IS PAHs in
toluene (200 ng/mL)
7.7
Syringe standard, 5-methyl-chyrsene (200 ng/mL)
in toluene
7.8
Spike solution of Petroleum crude oil (NIST 1582):
100 mg/mL in cyclohexane
8. Apparatus
Fisher Scientific USA
Part Number
8.1
Centrifuge, Heraeus
™
75-004-500
Multifuge
™
X3
8.2
Thermo Scientific 16 port SPE
03-251-252
vacuum manifold
8.3
Evaporator EVTM-130-32-16
3106395
(Fisher Scientific Germany)
8.4
Fisher precision balance
01918306
8.5
Vacuum pump
05-402-100
8.6
Rotavapor
®
R-210
05-024-21
8.7
Sartorius analytical balance
01-910-3224
8.8
Thermo Scientific Barnstead
0905050
EASYpure
™
II water
8.9
Ultrasonic bath Elmsonic S40H 154606Q
8.10
ULTRA-TURRAX
®
–
1425980
dispergation tool
8.11
ULTRA-TURRAX –
14259023
Plug-in coupling
8.12
ULTRA-TURRAX
142259301
8.13
Vortex shaker
14505141
8.14
Vortex standard cap
14-505-140
8.15
TSQ Quantum XLS Triple
Quadrupole Mass Spectrometer
9. Consumables
Part Number
9.1
GC vials
03393F
9.2
Pipette Finnpipette 100-1000 µL 14386320
9.3
Pipette Finnpipette 10-100 µL
14386318
9.4
Pipette Finnpipette 500-5000 µL 14386321
9.5
Pipette holder
14245160
9.6
Pipette Pasteur soda lime
136786A
glass 150 mm
9.7
Pipette suction device
03-692-350
9.8
Pipette tips 0.5 – 250 µL, 500/box
21377144
9.9
Pipette tips 1 – 5 mL, 75/box
2137750
9.10
Pipette tips 100 – 1000 µL,
2137746
200/box
9.11
Spatula, 18/10 steel
14356C
9.12
Spatula, nylon
NC9319088
9.13
SPE HyperSep SI,
03251270
200 mg/3 mL, 50 pc.
9.14
Tube holder
03840233
9.15
Wash bottle, PTFE
0340911A
9.16
Glass wool
386062500
9.17
GC column TR-50MS 30m,
260R142P
0.25 mm ID, 0.25 µm film
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