4
Figure 1: LC/MS analysis of 2AB labeled
N
-glycans from bovine fetuin using the GlycanPac AXH-1 column
Peak
Compound Stucture
(2AB labeling are not shown)
1
2
4
5
6
7
8
9
10
11a
11b
11c
12a
12b
13
14
15
Peak
Compound Stucture
(2AB labeling are not shown)
16
17
18
19
20
21
22
23
24
25
26
Table 1: Structural characterization of glycans present in each peak by the separation of 2AB labeled
N
-glycans
from bovine fetuin using a GlycanPac AXH-1 column
N
-acetyl
glucosamine
(GlcNAc)
Mannose
(Man)
Galactose
(Gal)
N
-acetyl
neuraminic
acid
(Neu5Ac)
N
-glycolyl
neuraminic
acid
(Neu5Gc)
L
-fucose
(L-Fuc)
0
10
20
30
40
0
100
1
4
7
6
11a-c
12a-b
16
14
15
13
19
20
21
18
23
24
25
22
2 4
17
26
Relative Abundance
Neutral
Monosialylated
Disialylated
Trisialylated
Tetrasialylated
Pentasialylated
5
8
9
10
Minutes
