AN20786_E 06/13S
Application Note 20786
thermoscientific.com/glycanpac
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Conclusion
The GlycanPac AXH-1 column is a high-performance, silica-based HPLC column for simultaneous
separation of glycans by charge, size, and polarity. It is designed for high-resolution and high-
throughput analysis with unique selectivity for biologically important glycans. We have
demonstrated that this column provides unique selectivity and excellent resolution for glycans
released from fetuin.
LC-MS/MS analysis of 2AB labeled
N
-glycan
s
derived from glycoproteins are demonstrated using
GlycanPac AXH-1 columns. The Q Exactive hybrid quadrupole-Orbitrap instrument provides
excellent MS/MS fragmentation information to enable characterization of glycan structures.
Reference
[1] Varki, A. Biological Roles of Oligosaccharides: All the theories are correct.
Glycobiology
,
1993
,
3
, 97–130.
[2] Bertozzi, C. R., Freeze, H.H., Varki, A. and Esko, J.D. Glycans in Biotechnology and the
Pharmaceutical Industry, Essentials of Glycobiology. 2nd edition. Chapter 51 Cold Spring
Harbor (NY): Cold Spring Harbor Laboratory Press; 2009.
[3] Guidance for Industry, Scientific Considerations in Demonstrating Biosimilarity to a Reference
Product, 2012, U.S.
/
GuidanceComplianceRegulatoryInformation/Guidances/UCM291128.pdf)
[4] Thermo Scientific Accucore HPLC Columns Technical Guide:
/
accucore.
[5] Thermo Scientific Accucore 150-Amide-HILIC application note on “Analysis of Human IgG
Glycans on a Solid Core Amide HILIC Stationary Phase”:
.
com/documents/AN-LC-Accucore-HILIC-Human-IgG-Glycans-AN20703_E.pdf
[6] Thermo Scientific GlycanPac AXH-1 Product Specification:
/
webdocs/114170-PS-GlycanPac-AXH1-Column-PS20695_E.pdf
[7] Bigge, J. C. et al., Non-selective and efficient fluroscent labeling of glycans using 2-amino
benzamide and anthranilic acid.
Analytical Biochemistry
,
1995
,
230
, 229-238.
[8] Apte, A and Meitei, N.S., Bioinformatics in glycomics: glycan characterization with mass
spectrometric data using SimGlycan
®
software,
Meth. in Mol. Biol
.,
2010
,
600
, 269-81.
