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Targeted Multiplexed Protein Quantitation Using Serial Immunoaffinity Extraction Coupled to LC-MS
Conclusion
MSIA D.A.R.T. extraction increases options for developing targeted
protein quantitation and transition into routine, analysis by:
Non-disruptive sample extraction process facilitates serial
extraction using different MSIA D. A.R.T. tips.
Serial extraction facilitates efficient multiplexing strategies on
low sample volume.
Serial extraction using same Ab (pooling) increases target
protein characterization, including N- and O-linked
glycopeptide/glycoform determinations.
Offline immunoaffinity extraction increases throughput by
reducing matrix, enriching signal, and facilitating multiplexed
analysis of pooled samples.
References
1. Nedelkov, D., Kiernan, U. A., Niederkofler, E. E., Tubbs, K. A.,
Nelson, R. W. PNAS, 2005, 102(31), 10852-10857
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lysis of peptide quantitation as a
pooled response for an unlabeled
copeptide. The response is
tide as a function of order
are boxed in Fig. 2c above.
C*GLVPLAENYN[Hex5HexNAc4NeuAc2]K
20
25
30
35
ides N- to C-terminus
Single2:Single3
Single2:Single4
Single2:Single5
Single2:Single6
response for all targeted
valuate the AUC ratio per peptide
ction across all subsequent MSIA