369 / 658
3
Thermo Scientific Poster Note
•
PN64316-EN 1114S
titative screening of drugs of
ometry.
e and analyzed as dried blood
. High resolution and accurate
FIGURE 1. Prototype paper spray ion source (Prosolia, Inc., IN) showing,
clockwise from top left: paper spray ion source, mechanism for dispensing
solvent to the sample, paper cassette indicating sample deposition and DBS-
spotted paper cassette electrospraying into mass spectrometer inlet.
Results
Screening for drugs of abus
identification
• Figure 2 shows that high an
iments for the identification and
o Scientific™ TraceFinder™
d spots at a 100 ng/mL level
d blood spots with this
at
m/z
200) are required wh
sample preparation and no
ppm at the higher resolving
• Results from TraceFinder s
screening analysis, are neat
y is easy to use, requires no
r a quick technique with the
ientific™ Orbitrap™ Exactive™
to paper spray ionization.
identified from a dry blood s
Screening for drugs of abus
• A drug mixture of six compo
forensic toxicology screenin
e mass spectrometric analysis
fore attractive for forensic
tion and storage of DBS in a
f
cocaethylene, codeine and
• This group of samples were
4) (140,000 resolving power
ensic toxicology lab sa e and
all molecules from complex
without time consuming sample
ell established even though a
Fragmentation and isotopic
• Accurate mass m/z values
pattern matching and two fr
confirmation (TraceFinder t
mass spectrometer can be
work used a Thermo Scientific
c MS/MS transitions, full-MS
id screening tools. Orbitrap
analysis for high confidence
ethod and retrospective data
• Figure 5 shows accurate m
DBS sample containing a m
resolution for enhanced sig
signal to noise is exception
demonstrated.
in addition to All Ion
sitive and fast Orbitrap
screening tool
Quantitation
• Amitriptyline-spiked in blood
25 ng/mL using amitriptyline
.
vine blood Lampire
FIGURE 2. Full scan MS experiments - highest resolving powers,
e.g.
, 70,000 and
140,000 (FWHM at
m/z
200), are required for the identification of drugs from DBS
due to matrix interference. Bovine blood spiked with six drugs, four drugs shown
below Resolving power from 17 500 35 000 70 000 and 140 000 top to bottom
• Variability in terms of %RSD
blood. Figure 6 displays ami
FIGURE 3. TraceFinder 3.0 s
targeted screening analysis
id tifi d b
t /
l
,
sample integrity maintained by
paper cartridges, dried under a
t hold up to 40 cassettes.
.
,
, ,
, ,
,
.
The [M+H]
+
ion is highlighted by a red line. Mass accuracies 1-2 ppm.
en e y exac m z va u
presence of two fragments f
Data collected with the Exa
lysis and an applied high
f the paper (Figure 1).
anol/water with 100 ppm acetic
244.2284
244.2069
300.1592
300.2911
318.1711
318.1964
318 1391
60
80
100 136.0738
amphetamine
PCP
codeine
cocaethylene
17 500
RP
Scientific™ Exactive Plus™ or
ometer.
f 30 sec data collection,
e Plus MS) or full scan and
244.2071
244.2269
300.2190
300.1614
300.1810
300.2907
300 2186
.
318.2667
318.1716
318.1916
40
60
80
100
tive Abundance
0
20
40
136.1130
136.0738
136.1127
136.1055
,
35,000
/MS (Q Exactive MS).
d fragmentation data were
. Normalized collision energy
244.2068
244.2268
.
300.1606
300.2912
300.1794
300.1433
318.1432
318.2648
318.1715
318.1414 318 2840
318.2286
0
20
40
60
80
100
0
20
Rela
136.1127
136.0739
136.1057
70,000
r™ sequences and contact
alibur platform was used for
244.15 244.20 244.25
244.2066
244.2269
300.1 300.2 300.3
300.1608
300.2910
300.2185
300.1431
300.3274
318.1 318.2 318.3
.
318.1711
318.1417318.1907318.2836
136.10
0
20
40
60
80
100
136.1127
136.0739
136.1056
136.0845
140,000
for the automated identification
m/z
m/z
m/z
m/z