LC-MS Applications for Environmental Analysis

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Application Note 1051
AN70386_E 04/13M
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Conclusion
This work describes a method that uses on-line SPE
HPLC with UV absorbance for determining paraquat
and diquat in drinking and environmental waters in
<10 min per sample. The determination is performed on
an UltiMate 3000 x2 Dual RSLC system controlled by
Chromeleon software. The reduced MDLs achieved
using UV detection and on-line SPE provide a convenient
method for determining these compounds in drinking
and environmental waters. This approach also meets the
MDL requirements specified in both U.S. EPA Method
549.2 and EU 98/83/EC.
References
1. Basic Information about Regulated Drinking Water
Contaminants; U.S. Environmental Protection
Agency, Environmental Monitoring and Support
Laboratory: Cincinnati, OH, 1997. [Online] http://
water.epa.gov/drink/contaminants/basicinformation/
diquat.cfm. (accessed Jan 28, 2013).
2. Directive 98/83/EC. Official Journal of the European
Council, Quality of Water Intended for Human
Consumption; L 330/42; 1998.
3. Method 549.2: Determination of Diquat and Paraquat
in Drinking Water by Liquid-Solid Extraction and
High-Performance Liquid Chromatography with
Ultraviolet Detection ; Revision 1.0; U.S. Environmental
Protection Agency, Environmental Monitoring and
Support Laboratory: Cincinnati, OH, 1997.
4. Thermo Scientific Application Brief 114: Improved
Separation of Diquat and Paraquat Using the Acclaim
Mixed-Mode HILIC-1 Column. Sunnyvale, CA, 2012.
[Online]
87647-AB114-LC-Diquat-Paraquat-Acclaim-HILIC-
1-05Oct2012-AB70230_E.pdf (accessed Jan 30, 2013).
5. Dionex (now part of Thermo Scientific) Application
Note 274: Sensitive On-Line SPE–HPLC Determination
of Paraquat and Diquat in Drinking and Environmental
Waters. Sunnyvale, CA, 2011. [Online] .
com/en-us/webdocs/110577-AN274-HPLC-Paraquat-
Diquat-Water-05Apr2011-LPN2726.pdf (accessed
Jan 30, 2013).
6. Product Specifications: Thermo Scientific Acclaim
Trinity Q1 Columns for Trace Analysis of Diquat
and Paraquat; PS20567_E 07/12S; Thermo Scientific:
Sunnyvale, CA, 2012. [Online]
en-us/webdocs/113757-PS-Acclaim-Trinity-
Q1-Jul2012-PS20567_E.pdf (accessed Jan 30, 2013).