LC-MS Applications for Environmental Analysis

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Results and Discussion
The triazine compounds eluted from the LC column in
20 minutes. A chromatogram of each compound and the
internal standards is shown in Figure 1. All peaks are
chromatographically resolved from one another. Calibration
curves were generated for each compound over the range
0.25-5 ppb. All calibration curves exhibited excellent
linearity, ranging from 0.9964 for Atrazine-desethyl to
0.9982 for Atrazine. The calibration curve for Simazine is
shown in Figure 2. The other compounds exhibit similar
linearity, and are not shown in this application note.
Conclusion
The TSQ Quantum Access LC-MS/MS is an excellent
choice for the analysis of triazine compounds and their
degradates. Linearity over the entire calibration range of
0.25 to 5 ppb is observed. Separation of all the analytes is
achieved with the Hypersil GOLD column allowing for
unambiguous identification and quantitation of all of the
compounds in this application note.
References
1. Smith, G.A., Pepich, B.V., Munch, D.J. “Determination of Triazine
Pesticides and their Degradates in Drinking Water by Liquid
Chromatography Electrospray Ionization Mass Spectrometry
(LC/ESI/MS)” Draft 5.0, April 2007.
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Figure 1: Chromatogram of the triazine compounds at 2 ppb, and their
internal standards
Figure 2: Calibration curve for Simazine, 0.25-5 ppb