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3
Sensitivity
Based on the Japanese Water Quality Standards Ordinance,
the method needs to be able to detect three regulated
haloacetic acids at concentration levels ten times lower than
the regulated amounts. Among the three haloacetic acids,
the compound MCAA has lowest regulated amount at
20 µg/L. The HAA column method was able to confirm all
three haloacetic acids at the 2 µg/L level, which is ten times
lower than 20 µg/L (Figures 3 and 4).
1
0
2
4
6
8
10
12
14
16
18
20
22
24
26
28
30
Time (min)
2
3
a
b
c
d
RT:
5.21 - 9.40
SM:
7G
6 7 8 9
Time (min)
0
20
40
60
80
100
Relative Abundance
RT: 7.30
SN: 23
NL:
2.42E3
TIC F: - c ESI
SRM ms2
93.000
[34.999-35.001]
MS Genesis
25Jun005
RT:
12.63 - 16.82
SM:
7G
14
16
Time (min)
0
20
40
60
80
100
Relative Abundance
RT: 14.83
SN: 133
NL:
1.31E5
TIC F: - c ESI
SRM ms2
127.000
[82.999-83.001]
MS Genesis
25Jun005
RT:
18.23 - 22.41
SM:
7G
20
22
Time (min)
0
20
40
60
80
100
Relative Abundance
RT: 20.36
SN: 496
NL:
1.49E5
TIC F: - c ESI
SRM ms2
161.000
[116.999-
117.001] MS
Genesis
25Jun005
a)
b)
c)
Figure 3. SRM chromatograms for 2 µg/L standard solution: a) MCAA, b) DCAA, c) TCAA
Figure 4. SRM chromatograms for tap water spiked with 2 µg/L standard solution: a) MCAA, b) DCAA, c) TCAA
*A peak from contaminant compound sources was detected before the peak in monochloroacetic acid. The identity of this
contaminant as a chloride ion cluster ion (NaCl
2
- ) was confirmed using a Thermo Scientific accurate-mass MS.
RT:
5.21 - 9.40
SM:
7G
6
7
8
9
Time (min)
0
20
40
60
80
100
Relative Abundance
RT: 7.20
SN: 14
6.07
8.82
NL:
2.69E3
TIC F: - c ESI
SRM ms2
93.000
[34.999-
35.001] MS
25Jun046
RT:
12.63 - 16.82
SM:
7G
14
16
Time (min)
0
20
40
60
80
100
Relative Abundance
RT: 14.76
SN: 482
NL:
2.68E5
TIC F: - c ESI
SRM ms2
127.000
[82.999-83.001]
MS Genesis
25Jun046
RT:
18.23 - 22.41
SM:
7G
20
22
Time (min)
0
20
40
60
80
100
Relative Abundance
RT: 20.19
SN: 807
NL:
2.92E5
TIC F: - c ESI
SRM ms2
161.000
[116.999-
117.001] MS
Genesis
25Jun046
Figure 2. Separation on the Acclaim HAA column. Upper section: Haloacetic acids 1: Monochloroacetic acid
(MCAA), 2: Dichloroacetic acid (DCAA), 3: Trichloroacetic acid (TCAA). Lower section: Matrix components
a: Ascorbic acid, b: Chloride, c: Nitrate, d: Sulfate