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Conclusion
It could be shown, that with DIA the sensitivity and
selectivity of fragment ions can be increased significantly.
Since this is a technique without targeted precursor ion
selection, no signals are filtered out. This enables all
possibilities for post acquisition processing in a targeted
and untargeted approach the same way, so prerequisites
for General Unknown Screening are given without
compromise.
References
1. B. Vogler, Master Thesis, University of Zurich,
Switzerland, 2013
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This information is not intended to encourage use of these products in any manners that might infringe the intellectual
property rights of others.
Presented at RAFA, Prague, Czech Republic, Nov5-8, 2013.
nts, starting with the
whole mass range
riment divided the
e following
to an increasing
nding up with eight
lation width of 50 Da
er of DIA windows on
nts on the example of
1.5
2.0
Time (min)
1.33
1.34
1.35
9
1.36
1.39
1.41
1.44
1.52
1.60
1.32
1.33
1 1.36
1.38
1.40
1.44
1.45
1.52
2.10
1.32
1.33
1
9 1.34
1.36
1.37
1.40
1.47
1.56
1.77
1.32
1 1.34
1.35
8
1.36
1.39
1.42
1.47
1.56
1.78
1.33
9
1.34
1.36
1.39
1.45
1.50
1.60
1.32
9
1.35
7
1.38
1.39
1.44
1.54
2.08
NL: 1.19E8
m/z=
286.1409-286.1467
F: FTMS +p ESI Full
ms [100.00-1000.00]
MS 130516pos_008
NL: 8.52E5
m/z=
268.1306-268.1360
F: FTMS +p ESI Full
ms2 MS
130516pos_008
NL: 4.61E6
m/z=
201.0892-201.0932
F: FTMS +p ESI Full
ms2 MS
130516pos_008
NL: 3.09E6
m/z=
229.0838-229.0884
F: FTMS +p ESI Full
ms2 MS
130516pos_008
NL: 1.88E6
m/z=
211.0734-211.0776
F: FTMS +p ESI Full
ms2 MS
130516pos_008
NL: 1.30E6
m/z=
183.0787-183.0823
F: FTMS +p ESI Full
ms2 MS
130516pos_008
RT:
0.69 -2.12
1.0
1.5
2.0
Time (min)
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
Relative Abundance
0
20
40
60
80
100
0
20
40
60
80
100
1.31 1.33
1.30
1.34
1.29
1.27
1.36
1.26
1.39
1.25
1.41
1.47
1.23
1.54
1.76
1.21
1.32
1.31
1.34
1.30
1.35
1.27
1.37
1.38
1.25
1.41
1.48
1.23
1.80
1.32
1.31
1.34
1.30
1.27 1.35
1.37
1.25
1.39
1.24
1.44
1.23
1.51
1.58
1.20
1.79
0.89
1.30 1.32
1.28
1.27 1.35
1.25 1.38
1.41
1.24
1.48
1.23
1.57 1.71
1.20
1.32
1.31
1.34
1.27
1.37
1.25
1.39
1.24
1.44
1.51
1.23
2.08
1.30 1.32
1.28
1.35
1.27
1.37
1.25
1.41
1.24
1.50
1.23
1.55 1.71
NL: 1.21E8
m/z=
286.1409-286.1467
F: FTMS + pESI Full
ms [100.00-1000.00]
MS 130516pos_010
NL: 9.15E5
m/z=
268.1306-268.1360
F: FTMS + pESI Full
ms2 MS
130516pos_010
NL: 3.73E6
m/z=
201.0892-201.0932
F: FTMS + pESI Full
ms2 MS
130516pos_010
NL: 2.64E6
m/z=
229.0838-229.0884
F: FTMS + pESI Full
ms2 MS
130516pos_010
NL: 1.71E6
m/z=
211.0734-211.0776
F: FTMS + pESI Full
ms2 MS
130516pos_010
NL: 1.12E6
m/z=
183.0787-183.0823
F: FTMS + pESI Full
ms2 MS
130516pos_010
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