123 / 302
3
Thermo Scientific Poster Note
•
PN21027 ISC 0814S
LC-
HRMS was performed using a Thermo Scientific™
QExactive
TM
system (Thermo Scientific, Bremen,
Germany), and the chromatographic and MS conditions
are detailed in Table 1.
Column
Thermo Scientific™
Accucore
TM
C18 2.6
m,
150 x 2.1 mm
LC
Mobile Phase A: 90:10 Aqueous 10mM Ammonium
Acetate : Acetonitrile
B: 20:80 Aqueous 10 mM Ammonium
Acetate : Acetonitrile
Injection volume
20
μ
L
Flow Rate
400
μ
L/min
HRMS
Capillary Temp
(
°
C)
350
Heater Temp
(
°
C)
300
Spray Voltage +ve. 4.5 kV
-ve. 3 kV
Capillary
Voltage
+ve. 52.5 V
-ve. 52.5 V
Tube Lens
Voltage
+ve. 135 V
-ve. 135 V
Resolution
50,000 FWHM
Scan Range
m/z: 100-1000
AGC Target
1,000,000
Max. Inject Time
100 ms
Fragmentation
Mode
HCD (20eV)
Week-long qua
influent showe
compounds we
respectively. A
0
5
0
50
100
0
50
100
0
50
100
% Relative Abundance
0
t
R
:
AA:
AH:
BP:
t
R
AA
AH
BP
t
R
AA
AH
BP
FIGURE 4. W
Thames river
1E+03
1E+04
1E+05
1E+06
1E+07
Wed T
Peak Intensity
TABLE 1. Chromatographic and MS experimental
conditions.