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Introduction

HyperSep SPE Phases

HyperSep Retain PEP

Polystyrene divinylbenzene material surface modified with

urea groups

Surface area 550 to 750m

2

/g

Particle size 40 to 60µm

Pore size 55 to 90Å

A versatile polymeric material for the retention of polar and non-polar

analytes ideal for applications such as drugs and metabolites in

biological matrices, environmental samples and desalting of peptides

in serum, plasma or biological fluids.

Mixed-Mode Phases

Two functional groups

Non-polar and ion exchange

Hydrophobic and ionic retention

Ideal for samples with complex structures

HyperSep Retain-CX

Versatile polymeric material for retention of basic compounds

Surface area 550 to 750m

2

/g

Particle size 40 to 60µm

Pore size 55 to 90Å

Retain-CX is a versatile polymeric material for the enhanced retention

of basic compounds. Typical application areas include the analysis of a

wide range of drugs of abuse from biological matrices.

HyperSep Retain-AX

Versatile polymeric material for retention of acidic compounds

Surface area 550 to 750m

2

/g

Particle size 40 to 60µm

Pore size 55 to 90Å

Retain-AX is a versatile polymeric material for the enhanced retention

of acidic compounds. Typical application areas include the analysis of a

range of acidic drugs of abuse from biological matrices, such as THC

and its metabolites.

HyperSep Hypercarb

Unique material for retention of highly polar compounds

100% porous graphitic carbon material

Retention of extremely polar compounds

Retention properties allow low bed weights

Provides total pH stability and the retention and separation of highly

polar species. It is ideal for problem analytes in SPE applications.

Reversed Phase Non-polar (Hydrophobic) Phases

Non-polar-non-polar interactions

Van der Waals or dispersive forces

PO LYME R I CS

olymerics

Reversed Phase Hydrophobic Phases

Non-polar-non-polar interactions

Van der Waals or dispersion force

HyperSep C18

Highly retentive alkyl-bonded silica phase for non-polar to

moderately polar compounds

Surface area 470 to 530m

2

/g

Particle size 40 to 60µm

Pore size 60Å

A silica-based material for applications such as drugs and their

metabolites in biological matrices, trace organics in environmental

water samples and toxins in food samples.

HyperSep C8

Less retentive alternative to C18 for non-polar to moderately

polar compounds

Surface area 470 to 530m

2

/g

Particle size 40 to 60µm

Pore size 60Å

A silica-based material for applications such as drugs and their

metabolites in biological matrices, trace organics in environmental

water samples, and toxins in food samples. C8 is used for hydrophobic

compounds which tend to be retentive on C18 columns.

HyperSep Phenyl

Alternative selectivity for retention of basic compounds

Surface area 470 to 530m

2

/g

Particle size 40 to 60µm

Pore size 60Å

Phenyl is a silica-based material which offers alternative selectivity for

aromatic compounds due to the presence of the benzene ring within the

structure. Typical applications include benzodiazepines in biological

matrices and extraction of aromatic compounds.

Reversed Phase

Silica Phases