AN20734_E 05/13S
Application Note 20734
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Conclusion
HyperSep Hypercarb SPE cartridges offer high levels of reproducibility as well as cleaner extracts,
which yields very good results. TraceGOLD TG-WaxMS GC columns are suitable for the GC-MS
analysis of acrylamide because of the low bleed stationary phase and better retention of polar
analytes compared with lower polarity stationary phases.
Reference
[1] Acrylamide in Drinking-water - Background document for development of WHO Guidelines
for Drinking-water, 2011,
/
acrylamide.pdf
Specified
Concentration
(µg/mL)
Calculated Concentration % Difference
0.25
0.225
-9.83
0.50
0.469
-6.22
1.00
0.983
-1.70
2.50
2.520
0.79
5.00
4.979
-0.41
10.0
10.037
0.37
Table 1: Accuracy data for the standard addition calibration curve for spiked
acrylamide in potato chips
Figure 1: TIC of chromatogram of 1 µg/mL spiked acrylamide (
m/z
71) and acrylamide-d
3
(
m/z
74) extracted from
potato chips
Time (min)
9.0
10
0
20
30
40
50
60
70
80
90
9.5
10.0
10.5
11.0
100
Relative Abundance
10
0
20
30
40
50
60
70
80
90
100
Acrylamide
Acrylamide-d
10.05 min
Impurity
Impurity
3
