4
Results
All measurements were carried out using the above
described instrumental setup with a co-injection of
sorbitol as analyte protectant. Symmetrical peak shapes
for all compounds of interest, including the critical pair
capsaicin and dihydrocapsacin, could be achieved, as
shown in Figure 4. The individual peaks for selected
compounds of the calibration runs, normalized to 100%
each, are given in Figures 5-8. The linear quantitative
calibrations with a zoom into the low concentration
range of 10-200 ppb are shown in Figure 9.
14.0
14.2
14.4
14.6
14.8
15.0
15.2
15.4
Time (min)
0
50
100
RT: 14.51
AA: 79906
RT: 14.77
AA: 72753
14.0
14.2
14.4
14.6
14.8
15.0
15.2
15.4
Time (min)
0
50
100
RT: 14.49
AA: 27108
RT: 14.75
AA: 24730
14.79
14.54
14.84
15.14
14.94
14.06
15.21
14.35
14.14
10 ppb
Capsaicin
Dihydrocapsacin
(A)
(B)
Figure 4. Capsaicin and dihydrocapsacin peak shape, (A) without and (B) with analyte
protectant, both runs at 10 ppb concentration, 30 m column of Figure. 3.
7.8
7.9
8.0
8.1
Time (min)
0
20
40
60
80
100
Relative Abundance
RT: 7.92
AA: 96993015
SN: 82873
RT: 7.86
AA: 1791377
SN: 1561
RT: 7.92
AA: 8920897
SN: 18416
RT: 7.93
AA: 913005
SN: 1913
10 ppb
100 ppb
200 ppb
1000 ppb
Figure 5. Thymol calibration peaks 10-1000 ppb.
8.0
8.1
8.2
8.3
8.4
8.5
Time (min)
0
20
40
60
80
100
Relative Abundance
RT: 8.24
AA: 33817857
SN: 825929
RT: 8.24
AA: 6346374
SN: 310523
RT: 8.24
AA: 2972548
SN: 221745
RT: 8.27
AA: 83321
SN: 924
RT: 8.24
AA: 338618
SN: 13200
10 ppb
100 ppb
200 ppb
1000 ppb
Figure 6.
α
-Methyl-trans-cinnamaldehyde calibration peaks 10-1000 ppb.
Figure. 7. Capsaicin calibration peaks 10-1000 ppb.
Figure 8. Piperine calibration peaks 10-1000 ppb.
14.0
14.2
14.4
14.6
Time (min)
0
20
40
60
80
100
Relative Abundance
RT:14.50
AA:23410769
SN:38532
RT:14.66
AA:1029630
SN:777
RT:14.50
AA:3808879
SN:10960
RT:14.66
AA:189269
SN:301
RT:14.50
AA:1859072
SN:8500
RT:14.66
AA:90016
SN:213
RT:14.51
AA:203012
SN:1352
10 ppb
100 ppb
200 ppb
1000 ppb
16.3
16.4
16.5
16.6
Time (min)
0
20
40
60
80
100
Relative Abundance
RT: 16.49
AA: 8088783
SN: 12182
RT: 16.49
AA: 1341210
SN: 5366
RT: 16.49
AA: 665343
SN: 1975
RT: 16.50
AA: 66903
SN: 260
10 ppb
100 ppb
200 ppb
1000 ppb
