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Multiplexing Multiple Methods to Maximize Workflow Efficiency in LC-MS Laboratories
forensic
ls of a
(UHPLC)
) in order
tes with
up to
d serum
nd
al
nds:
is and
ting into a
ability
ecimen
C system.
ss than
nd
ntion time
lculated
eries on a
ent ion
e
es and
into one
fferent
s and
effort.
Results
Multi-channeling batches of 25-OH-VitDs and MMA
FIGURE 1. Common APCI Source Conditions
FIGURE 2. MS/MS & LC Conditions for 25-OH-VitDs
Start data 1.1 min
Data window: 1.0 min
Total run time: 4.0 min
FIGURE 3. Typical results for 25-OH-VitDs
Desired quantitation range from 4 to 80 ng/mL achieved
25-OH-VitDs batches submitted to one or two channels have
throughputs of 15 or 28 injections per hour, respectively. Large
batches submitted across 4 channels typically have throughputs
around 58 injections per hour.
FIGURE 4. Multi-channeling 25-OH-VitDs
Multi-c
FIGUR
FIGUR
Sta
FIGUR
Desired
FIGUR
results.
System Control & Data Analysis
Thermo Scientific™
TraceFinder
™ with Aria™ MX software was used
to control the Transcend II LX4 and Endura MS/MS systems, submit
batches to desired channels as well as for analyzing data and
reporting results.
The caus
calculate
measure