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Quantitative Analysis of THC and Main Metabolites in Whole Blood Using Tandem Mass Spectrometry and Automated Online Sample Preparation
Limits of quantification were determined as the lowest concentration for which a 20%
RSD is obtained as well as a bias inferior to 20%. The results are presented on figure9.
The limits of quantification satisfy the requirements for cannabis analysis in whole
blood, considering that the limits of detection are expected to be close to 0.5 ng/mL.
Conclusion
A fast, automated, and analytically sensitive LC-MS/MS method was developed to
quantify THC and its metabolites in crashed whole blood.
The total online extraction and analytical LC runtime was 10.4 minutes. This
throughput could be increased by multiplexing this method on a Thermo Scientific
Transcend TLX system.
This method was linear from 0.5 to 100 ng/mL.
The lower limit of quantitation was at least of 0.5 ng/mL for THC
and its
metabolites. Good repeatability was obtained for the different calibration levels
with %RSD inferior to 10%.
Correlation between GC-MS and this analytical method is being performed by
Analysis
–
Expertise laboratory.
References
1. Musshoff F., Burkhard M.
–
Therapeutic Drug Monitoring, 28 (2006) 155-163
2. Fabritius M., Staub C., Giroud C.
–
Annales de Toxicologie Analytique 23 (2011)
21-35
3. Peters F., Drummer O., Musshoff F.
–
Forensic Science International 165 (2007)
216-224
Acknowledgements
We would like to thank Dr Christophe Petit from Analysis Expertise for supplying the
blood samples and the standards for this work.
1-OH-THC and THC-COOH from spiked
nges goes from 0.5ng/mL to 100ng/mL.
X
ual
tration (ng/mL)
qual
tration (ng/mL)
5 X
qual
tration (ng/mL)
s. The mean calculated concentration, the
SD) for each calibration point are presented
bility (%RSD) obtained for each
FIGURE 9. Limits of quantification for THC, OH-THC and THC-COOH in spiked
and crashed whole blood samples.
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