160 / 658
6
Versatile Solutions to Current Demands for Automated Sample Cleanup, High Throughput, and High-Resolution Chromatography on a Single LC-MS Platform
FIGURE 6. Versatility chart for Transcend II LX-4 system with Q Exactive hybrid
quadrupole-Orbitrap MS platform.
ns collected into a single
in (four injections).
RT:91.24
AA: 96646
SN: 329
64
929
RT:87.88
AA:93785
SN:324
RT:75.59
AA:93956
SN:318
RT:95.19
AA:92747
SN:311
RT:101.37
AA:90521
SN:305
RT:87.89
AA:603052
SN:1741
RT: 75.60
AA:608291
SN:1697
RT:93.48
AA:589245
SN:1651
RT:101.37
AA: 576524
SN: 1640
NL:
9.95E4
m/z=
862.59973-
862.61699
MS ICIS
Sample1537
NL:
6.13E5
m/z=
888.61512-
888.63290
MS ICIS
75
80
85
90
95
100 105
Sample1537
RT:53.82
AA:78593
SN:259
RT:54.07
AA: 80310
NL:
8.52E4
m/z=
862.59973-
86261699
SN:236
RT:54.02
AA: 9340
SN: 28
RT:53.82
AA:494857
SN:1347
RT:54.07
AA:523323
SN:1297
.
MS ICIS
Sample1537
NL:
5.11E5
m/z=
888.61512-
888.63290
MS ICIS
Sample1537
53.8
53.9
54.0
54.1
nup and chromatographic
dow of 30 s using a
c range investigated
,
all four
6 and 0.99. Internal standard
Conclusion
than
,
16% for each curve.
ata windows) resulted in the
baseline chromatographic
This work has effectively demonstrated the versatility of the Transcend II LX-4
with Q Exactive MS platform (Figure 6), which enables the user to perform fast
online sample cleanup as needed or standard HPLC. Additionally, collection of
multiple injections into a single data file can accomplish ultra-high sample
throughput, whether for research screening applications or for true
g with another sulfatide, was
ted into 3 single data files
n average of 2.2 samples per
chromatography. The use of high-resolution, accurate-mass data collection adds
in both the sensitivity and selectivity to the assay. The staggered injection
capabilities of the multi-channel Transcend II LX-4 LC with the Q Exactive MS
platform can be used to continuously acquire data during the window where the
compounds are eluting. QuickCalc software offers accurate data parsing of
those multiple injections into a single data file
file using 9 s data windows)
resolution by accurate mass
mmarized in Table 3.
ined %RSD of multiple
.
In conclusion, it is important to note that any high-throughput workflow
determinations must involve considerations to the requirements of the assay,
such as chromatographic separation of isobaric compounds, removal of matrix
interference, and carryover associated with either the LC column or the
ection of 384-sample batches
l T
d II LX 4 t
orkflow of ≤5.8% and with
s data window workflow of
autosampler. Each of these considerations may pose restrictions to highest
throughput. Overall, the greatest strength of this platform lies in the versatility
that it can bring to either clinical research or drug discovery laboratories.
e ranscen - sys em
Exactive HRAM MS.
Injections
References
1. Chassaing, C.; Robinson, S.;
Chromatography Today,
2
009
, September,
20-24.
hromatography
perminute
elineseparationof3
analytes
0.63
elineseparationof3
analytes
1.7
elineseparationof3
analytes
2.2
CTC is a trademark of CTC Analytics AG, Zwingen, Switzerland. All other trademarks are the property of Thermo
Fisher Scientific and its subsidiaries.
elutionof2analytes
4.0
For Research use only. Not for use in diagnostic procedures.
This information is not intended to encourage use of these products in any manners that might infringe the
intellectual property rights of others.
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