AB71410-EN-BEER-Compendium-0716S.pdf

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AB71410-EN 0716S-B10
Overview:
Mycotoxins are a group of naturally occurring chemicals produced by microscopic
filamentary fungi. They can grow on a variety of different cereals, including barley.
Reliable analytical methods for fast and effective monitoring of mycotoxins during the
beer production chain are essential.
The aim of this study was to introduce a method for analysis of 32 mycotoxins in beer,
based on very simple sample preparation and ultra high performance liquid
chromatography (UHPLC) coupled with full spectral Mass Spectrometry (MS)
detection.
Method:
Mycotoxin standards, including Fusarium toxins and aflatoxins were used. Beer
samples were treated to precipitate out contaminants. A Thermo Scientific™ Accela™
UHPLC system with a Thermo Scientific™ Hypersil GOLD™ aQ , 1.9µm 100 x 2.1mm
column, was used for the separation of target analytes. Detection was carried out
using a Thermo Scientific ™ Orbitrap ™ LC-MS system.
Column Part Number Description
25302-102130 Hypersil GOLD aQ 1.9µm 100 x 2.1mm
Conclusion:
The method enables rapid determination of trace levels of multiple mycotoxins
occurring in complex beer samples.
Multi-mycotoxin Screening and Quantitation Using
UHPLC, High Resolution and Accurate Mass
Milena Zachariášová, Jana Hajšlová, Institute of Chemical Technology, Prague, Czech Republic
Michal Godula, Thermo Fisher Scientific, Prague, Czech Republic
Application Summary AN 51961