AB71410-EN-BEER-Compendium-0716S.pdf

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AB71410-EN 0716S-B9
Overview:
In this study, two targeted assays were developed for the measurement of either
polyphenols (including catechins and proanthocyanidins) or xanthohumols and
bitter acids.
The bitter acid method was used to study beer stability. A metabolomic approach
was also developed where patterns of both known and unknown analytes were
used to study differences between beer samples–an approach that is relevant to
quality control.
Method:
A Thermo Scientific™ Dionex™ UltiMate™ 3000 System with a Thermo Scientific™
Acclaim™ 120, C18 3µm 3.0 x 150mm column. Detection was carried out using UV
(Thermo Scientific™ Dionex™ DAD-3000RS diode-array) and EC (Thermo Scientific™
Dionex™ CoulArray detector with thermal organizer). Data was analyzed using the
Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System version 6.8
and Thermo Scientific™ Dionex™ CoulArray ™ software 3.1. EC-array data were
transferred to Pirouette® software for chemometric analysis.
Column Part Number Description
063691 Acclaim 120 C18 3µm 3.0 x 150mm
Conclusion:
The polyphenol method employs a targeted approach to accurately and sensitively
measure various phenols, phenolic acids and polyphenols in beer, not possible by UV
alone. Metabolomic approaches using the patterns of numerous known and unknown
analytes can be used to differentiate between different samples. Such an approach
can be used to study fermentation, product stability, and authenticity.
Use of EC detection eliminated the need for solid phase extraction procedures for
sample pre-concentration commonly used in UV detection methods.
Chalconoids and Bitter Acids in Beer by
HPLC with UV and Electrochemical Detection
Paul A. Ullucci, David Thomas, and Ian N. Acworth
Thermo Fisher Scientific, Chelmsford, MA
Application Summary AN 1020