AB71410-EN-BEER-Compendium-0716S.pdf

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AB71410-EN 0716S-B8
Overview:
Nitrosamines is the common term used for compounds of the class of
N-nitrosodialkylamines. Nitrosamines in food are mainly produced from nitrites, added
to food as preservatives. Another source of nitrosamines is during the drying process
of germinated malt in beer production. Nitrosamine levels in malt and beer have been
significantly reduced in the brewing process, due to regular control, requiring high
analytical performance.
This application note describes a comprehensive GC-MS/MS method for the high
sensitivity detection of nitrosamine compounds at low levels.
Method:
Nitrosamines were isolated from beer samples using an adapted method from AOAC
Official Method (2000), 982.11. Chromatographic analysis was performed using a
Thermo Scientific™ TriPlus™ RSH Autosampler and a Thermo Scientific™ TRACE™ 1310
Gas Chromatograph with a Thermo Scientific™ TraceGOLD™ TG-Wax MS column
30m x 0.25mm x 0.5µm. All samples were analyzed on a Thermo Scientific™ TSQ
8000™ Triple Quadrupole GC-MS/MS system with the unique AutoSRM analysis
software. Data processing was carried out with Thermo Scientific™ TraceFinder™
software, for quantitation and targeted screening workflows.
Part Number Description
26088-2230 TraceGOLD TG-WaxMS 30m x 0.25mm x 0.5µm
31303233-BP BTO Septa 11mm in Blister Packaging
365D0291 TriPlus RSH Syringe 10µl FN 57mm 26s Gauge
Conclusion:
The TSQ 8000 GC-MS/MS can successfully quantify the concentration of nitrosamine
components in real samples without any uncertainty, and can serve as a turnkey
method for routine use in beer and food safety control. The presented method is fast,
allows high sample throughput and provides results with very high sensitivity.
High Sensitivity Analysis of Nitrosamines
Using GC-MS/MS
Alex Chen1, Hans-Joachim Huebschmann2, Li Fangyan 3, Chew Yai Foong3
and Chan Sheot Harn3
1Alpha Analytical Pte. Ltd., Singapore, 2Thermo Fisher Scientific, Singapore
3Health Sciences Authority, HSA Singapore
Application Summary AN 10315