Page 48 - BR20828_E Accucore 0713S
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TN20690_E 02/13S
Technical Note 20690
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Conclusion
The high efficiency of the Accucore XL HPLC column has been demonstrated. It has been
shown longer Accucore XL HPLC columns provide the greatest separation and peak capacity.
However, separation of a complex mixture can be maintained when decreasing the column
length to improve analysis times. The shortest Accucore XL HPLC column was shown to
provide the greatest number of peaks per minute without compromising the separation.
Therefore, the high resolution offered by the Accucore XL HPLC column can be used to
improve complex separations through an increase in peak capacity to analysis time ratio, which
makes it an ideal candidate for improving the overall performance of a separation.
References
[1] Giddings J. C. Maximum Number of Components Resolvable by Gel Filtration and Other
Elution Chromatographic Methods.
Anal
. Chem.
1967
,
39
, 1027.
[2] Gritti F. The Mass Transfer Mechanism of Columns Packed With sub-3 µm Shell Particles
and its Reproducibility for Low- and High-Molecular Weight Compounds.
Chromatography
Today
2012
,
5
(2), 4-11.
Peak 1
RT (min)
Peak 2
RT (min)
Resolution Selectivity
Rep 1 18.06 18.59
3.35
1.03
Rep 2 18.05 18.58
3.40
1.03
Rep 3 18.07 18.61
3.42
1.03
Average 18.06 18.59
3.39
1.03
%RSD 0.05
0.06
1.06
0.02
(a) 450 mm
Peak 1
RT (min)
Peak 2
RT (min)
Resolution Selectivity
Rep 1 12.62 12.97
2.83
1.03
Rep 2 12.65 13.00
2.72
1.03
Rep 3 12.64 12.99
2.73
1.03
Average 12.64 12.99
2.76
1.03
%RSD 0.16
0.09
2.20
0.06
(b) 300 mm
Peak 1
RT (min)
Peak 2
RT (min)
Resolution Selectivity
Rep 1 7.58
7.82
2.45
1.03
Rep 2 7.58
7.83
2.45
1.03
Rep 3 7.58
7.82
2.45
1.03
Average 7.58
7.83
2.45
1.03
%RSD 0.03
0.04
0.00
0.01
(c) 150 mm
complex samples. In addition, the selectivity between
the critical pair does not change with decreasing column
length, which indicates that the separation remains
reproducible for the analysis of this complex sample on
the different Accucore XL HPLC column lengths.
Statistical examination of the critical pair on the
different column lengths, seen in Tables 3a-c, show
that the data is matched with excellent precision.
Run-to-run reproducibility is of particular importance
when analyzing complex mixtures, as it enables the
examination of batch-to-batch differences between
Tables 3a-c: Statistical examination of the critical pair detected in the complex mixture for Accucore XL HPLC column lengths of
450 mm (a), 300 mm (b) and 150 mm (c)
