Page 47 - BR20828_E Accucore 0713S

3 ≤600 bar. This means, if necessary, for complex samples
highly efficient separations can be performed within the
pressure limits of conventional HPLC instrumentation.
Figure 1: Overlaid chromatograms for the separation of green tea extract on Accucore XL HPLC column lengths 150 mm (a),
300 mm (b), and 450 mm (c). The critical pair is circled on each chromatogram and the resolution for each critical pair is indicated.
There is low resolution for the early eluters on the 150 mm column (c), as the focus of the gradient is based on the critical pair.
mAU
min
0.0 5.0 10.0 15.0
(a)
(b)
(c)
Rs = 3.39 Accucore XL 4μm, 450 × 2.1mm (ΔP = 601 bar)
Rs = 2.76 Accucore XL 4μm, 300 × 2.1mm (ΔP = 430 bar)
Rs = 2.45 Accucore XL 4μm, 150 × 2.1mm (ΔP = 226 bar)
20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0
The lower backpressures generated on the Accucore XL
HPLC column allowed column lengths of up to 450 mm
to be used to maximize peak capacity with backpressures
column, the number of detectable peaks per minute (peak
capacity to analysis time ratio) increased by up to 50%,
as seen in Figure 2, with only a 28% loss in resolution
of the critical pair. This means that sample throughput
can be increased through a reduced analysis time without
significantly affecting the quality of the data produced
based on the critical pair.
Figure 2: The number of peaks per minute increases by up to 50% as
column length decreases by 67%. Therefore, shorter analysis times
with good resolution can be achieved using the shorter column.
Peak Capcity to Analysis Time Ratio
1.00
0.00
450 300 150
2.00
3.00
4.00
5.00
6.00
7.00
8.00
9.00
Column length (mm)
The Accucore XL HPLC column is shown to offer the
analyst a choice of either maximizing peak capacity
or sample throughput. Resolution and peak capacity
can be maximized using the longer 450 mm column, as
seen in Figure 1 and a summary of the peak capacities
achieved on each column is seen in Table 2. However, by
shortening the column length of the Accucore XL HPLC
Column Length (mm) Run Time (mins) Peak Capacity
150 20 167
300 40 280
450 60 336
Table 2: Summary of the peak capacities achieved on the different
Accucore XL HPLC column lengths