Page 57 - BR20828_E Accucore 0713S
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TN20668_E 01/13S
Technical Note 20668
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Conclusion
It has been demonstrated that the Accucore XL C8 HPLC column is stable following 2,200
injections of a solution containing the NSAIDS naproxen, fenoprofen, and ibuprofen. Using
isocratic conditions, full separation of the NSAIDs was achieved. The precision of capacity
factors and efficiency for the NSAIDs over the course of the investigation is excellent, and
the Accucore XL C8 HPLC column was shown to provide excellent peak shape for these
compounds.
The Accucore XL HPLC columns have been proven to remain stable over the course of the
analysis, which makes them ideal columns to choose to obtain high-performing and robust
HPLC separations.
Figure 2: Assessment of the reproducibility of retention for naproxen, fenoprofen, and
ibuprofen over 2,200 injections on the Accucore XL C8 HPLC column
0
500
1000
1500
2000
2500
Number of Injections
10
Capacity Factor (k')
9
8
7
6
5
4
3
2
1
0
Figure 3: Assessment of the reproducibility of efficiency for naproxen, fenoprofen, and
ibuprofen over 2,200 injections on the Accucore XL C8 HPLC column
0
500
1000
1500
2000
2500
Number of Injections
150%
Normalized Effciency
140%
130%
120%
110%
100%
90%
80%
70%
60%
50%
Stability - Retention
•
Ibuprofen
•
Fenoprofen
•
Naproxen
Stability - Efficiency
•
Ibuprofen
•
Fenoprofen
•
Naproxen
