Page 53 - BR20828_E Accucore 0713S
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TN20742_E 04/13S
Technical Note 20742
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mAU
Minutes
1
0
0
5
10
2
3
4
5
6
7
8
S/N = 4.9
S/N = 5.3
S/N = 10.6
Fully porous 5 μm
Fully porous 3 μm
Accucore XL 4 μm
Figure 4: Comparison of average signal-to-noise ratios (S/N) on fully porous 5 and 3 µm and Accucore XL 4 µm
columns for 1 ng of each solute loaded on column
Experimental conditions: columns – 150 × 4.6 mm; mobile phase – water and acetonitrile; gradient – 35%
to 60% acetonitrile in 7.5 min; flow rate – 1.3 mL/min; column temperature – 30 °C; UV detection – 247 nm;
injection volume – 5 μL; solutes – 1. Uracil, 2. Tebuthiuron; 3. Metoxuron; 4. Monuron; 5. Chlorotoluron; 6.
Diuron; 7. Linuron
Conclusion
• The solid core 4 μm particles in Accucore XL HPLC columns provide significant improvements
over fully porous 5 μm and 3 μm particles in terms of separation efficiency and sensitivity of the
analysis.
• The Accucore XL 4 µm columns significantly improve sensitivity over fully porous 5 μm
and 3 μm columns, with no changes to methodology or HPLC system configuration.
References
1. U. D. Neue, HPLC Columns: Theory, Technology, and Practice, Wiley-VCH, New York, 1997,
page 334
2. USP-32, Ibuprofen, Chromatographic Purity
