TN20667_E 01/13S
Technical Note 20667
thermoscientific.com/accucore
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Conclusion
• The Accucore XL 4 µm HPLC columns produce
significant resolution improvements over fully
porous 3 μm and 5 μm columns with no changes to
methodology or HPLC system configuration.
• The solid core 4 µm particles in Accucore XL HPLC
columns provide significant improvements over
fully porous 5 μm and 3 µm particles in terms of
separation efficiency and resolution.
• The flat nature of the van Deemter curves on the
Accucore XL 4 µm HPLC columns enable reduction
in analysis time by optimization of flow rate and
gradient conditions.
Figure 5: Productivity comparison for fully porous 5 µm and Accucore XL 4 µm HPLC columns. Experimental conditions: columns – C18,
150 x 4.6 mm; mobile phase – water and acetonitrile; standard gradient – 35% to 60% acetonitrile in 10 min and flow rate – 1 mL/min;
scaled gradient: 35% to 60% acetonitrile in 7.5 min and flow rate – 1.3 mL/min; optimized gradient: 35% to 60% acetonitrile in 4.5 min
and flow rate – 1.3 mL/min; column temperature – 30 °C; UV detection – 247 nm; injection volume – 5 μL
References
[1] Thermo Scientific Technical Note 20641: Solid Core
4 μm HPLC Column Comparison to Fully Porous 3 μm
and 5 μm Columns: Efficiency and Pressure.
Minutes
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
(a)
(b)
(c)
(d)
Rs = 2.47
Rs = 3.13
Rs = 3.02
Rs = 2.99
Rt = 4.06
Rt = 5.07
Rt = 6.56
Rt = 8.62
Fully porous 5 µm
Standard gradient
Accucore XL 4 µm
Standard gradient
Accucore XL 4 µm
Scaled gradient
Accucore XL 4 µm
Optimized gradient
1
Uracil
2
Tebuthiuron
3
Metoxuron
4
Monuron
5
Chlorotoluron
6
Diuron
7
Linuron
1...,33,34,35,36,37,38,39,40,41,42 44,45,46,47,48,49,50,51,52,53,...58