Page 33 - BR20828_E Accucore 0713S

TN20755_E 04/13S
Technical Note 20755
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Conclusion
Comparison of the Accucore 2.6 µm and Accucore XL 4 µm solid core columns shows that:
• The backpressure of the Accucore 2.6 µm column is 2.2 times higher.
• The Accucore 2.6 µm column is 20% more efficient per unit time.
• The Accucore 2.6 µm column has 37% lower impedance.
The choice between these two solid core materials should be based on the assay goals and
the equipment available. The Accucore XL 4 µm columns dramatically improve separation
efficiency, and therefore resolution and sensitivity over those obtained with conventional fully
porous 5 and 3 µm particle packed columns, without the need to make changes to the operating
parameters or system configuration [4]. As demonstrated above, the Accucore 2.6 µm columns
provide even higher efficiency and lower impedance, but often system dead volume and
operating parameters have to be optimized to get the best possible performance out of these
columns [5]. Additionally, when operating at the higher linear velocities, a 600 bar pressure
limit LC system may be required.
Therefore, Accucore XL 4 µm columns should be used when:
• There is large dead volume in the system.
• The maximum operating pressure of the pumps is 400 bar.
• The same method as used with a fully porous particle packed column must be maintained.
In contrast, Accucore 2.6 µm columns should be used when even higher efficiency is required
and:
• The dead volume of the system is minimal (<100 μL).
• The maximum operating pressure of the pumps is greater than 400 bar.
• The method can be optimized.
References
[1] T. Farkas, G. Zhong and G. Guichon, J. Chrom. A ., 1999, 849, 35.
[2] J.C. Giddings, Unified Separation Science, J. Wiley & Sons (1991) p. 65.
[3] G. Desmet, P. Gzil, D. Clicq, LC GC Europe, 2005 , 18, 403.
[4] Thermo Fisher Scientific, Technical Note 2064, 2012.
[5] L. Pereira, Chromatography Today, 2012, 5(2), 20.