3
Figure 4: Comparison of the steric selectivity and hydrophobic retention of the tested stationary phases
Steric selectivity
Hydrophobic retention
0.0
1.05
1.00
1.10
1.15
1.20
1.25
1.30
1.35
1.40
1.45
1.50
5.0
10.0
15.0
20.0
25.0
30.0
35.0
40.0
Accucore C18
Kinetex C18
Poroshell 120 SB-C18
Halo C18
Ascentis Express C18
Nucleoshell RP-C18
SunShell C18
Accucore RP-MS
Most hydrophobic
Figure 5: Radar plots for the phases tested, showing the differences in selectivity. HR = hydrophobic retention;
HS = hydrophobic selectivity; SS = steric selectivity; HBC = hydrogen bonding capacity; IEX (7.6) = ion exchange
capacity at pH 7.6; BA = activity towards bases; C = activity towards chelators; IEX (2.7) = ion exchange capacity
at pH 2.7; AI = activity towards acids
C
BA
IEX (2.7)
AI
HR /10
HS
SS
HBC
IEX (7.6)
Halo
C
BA
IEX (2.7)
AI
HR /10
HS
SS
HBC
IEX (7.6)
Nucleoshell
C
BA
IEX (2.7)
AI
HR /10
HS
SS
HBC
IEX (7.6)
Accucore RP-MS
C
BA
IEX (2.7)
AI
HR /10
HS
SS
HBC
IEX (7.6)
Accucore C18
C
BA
IEX (2.7)
AI
HR /10
HS
SS
HBC
IEX (7.6)
Sunshell
C
BA
IEX (2.7)
AI
HR /10
HS
SS
HBC
IEX (7.6)
Ascentis Express
C
BA
IEX (2.7)
AI
HR /10
HS
SS
HBC
IEX (7.6)
Poroshell 120
C
BA
IEX (2.7)
AI
HR /10
HS
SS
HBC
IEX (7.6)
Kinetex