Page 24 - BR20828_E Accucore 0713S

TN20788_E 06/13S
Technical Note 20788
thermoscientific.com/accucore
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Conclusion
The selectivities of the Accucore C18 and RP-MS phases were assessed against six other
comparable solid core reversed-phase stationary phases. The retention properties of the
stationary phases were categorized by analyzing primary modes of interaction (hydrophobicity,
steric selectivity, and hydrogen bonding) and secondary or unwanted modes of interaction (ion
exchange and chelation). The results were summarized in radar plots, which identified the
following main differences:
1) Primary interactions
• Halo C18, SunShell C18, and Nucleoshell RP-C18 are the most hydrophobic phases
and therefore the most retentive towards hydrophobic solutes. A varying degree of
hydrophobicity was observed throughout the materials, reflecting different degrees of
surface area and coverage of this silica surface.
• Accucore C18 shows the highest steric selectivity, achieved without excessive
retention. This attribute of the phase facilitates good separation of solutes that have similar
hydrophobicity but dissimilar steric structures.
2) Secondary interactions
• All phases show evidence of efficient endcapping and the absence of dissociated acidic
silanols.
• Poroshell 120 SB-C18 and SunShell C18 have the highest levels of chelation, indicating
the highest metals content; analysis of solutes which can chelate on these phases may result
in asymmetrical peaks.
• Poroshell 120 has the highest silanolic activity at neutral pH; this can cause tailing of
solutes capable of ion exchanging with silanols under these pH conditions.
• SunShell C18 has the highest activity towards acids and protonated bases, and therefore
asymmetrical peaks can be expected for these types of compounds on this phase.
References
[1] Tanaka, N. et al., Journal of Chromatographic Science, 1989, 27, 721-728.