Page 18 - BR20828_E Accucore 0713S

18 Thermo Scientific Accucore LC Columns | Technical Notes
Parameter Term
HR Hydrophobic Reten-
tion Retention of compounds based on their hydrophobicity k'
HS Hydrophobic
Selectivity
Separation of compounds that have similar structure,
but differ slightly in hydrophobicity α
SS Steric
Selectivity
Separation of compounds that have similar structure,
but differ in shape α
HBC Hydrogen Bonding
Capacity Separation related to degree of end capping α
T1: Hydrophobic Interactions
Parameter Term
BA Base
Activity
Peak shape for basic analytes resulting from total silanol activity
(all dissociated at pH 7.6) t f
C Chelation Peak shapes for chelating analytes resulting from silica
metal content t f
IEX(7.6) Ion Exchange
Capacity (pH 7.6)
Separation between basic and neutral compounds resulting from
total silanol activity (all dissociated at pH 7.6) α
T2: Secondary Interactions Under Neutral pH
Parameter Term
AI Acid
Interaction Interactions resulting in poor peak shape for acidic analytes t f
IEX(2.7) Ion Exchange
Capacity (pH 2.7)
Separation between basic and neutral compounds resulting from
acidic silanol activity α
T3: Secondary Interactions Under Acidic pH
Optimum Selectivity
Accucore based on 2.6 μm particles is available in fourteen different phases to provide an unrivalled
range of selectivities.
Each of the bonded phases is manufactured using advanced bonding technology and is characterized
using a testing regime based on the Tanaka Tests.