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Thermo Scientific Poster Note
•
PN64227-MSACL-EN 0814S
Concl
Multi-c
efficien
Multi-c
mass s
Ackno
We thank
Park, NJ) f
on desired
one
ent
nd
fort.
ality
g 1:2
3-d6 or
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acetyl
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ctions
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uL of
-d4 for
uL of
ation.
ade
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ining
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ientific.
umps
obile
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h MMA
the
around 58 injections per hour.
FIGURE 4. Multi-channeling 25-OH-VitDs
FIGURE 5. MS/MS & LC Conditions for Butyl-MMA
Start data 1.75 min
Data window: 1.0 min
Total run time: 5.0 min
FIGURE 6. Typical results for MMA (butylated)
Desired quantitation range from 0.05 to 1.00 uM achieved
MMA batches submitted to one or two channels have throughputs of
12 or 23 injections per hour, respectively. Since demand for MMA is
much less than for 25-OH-VitDs, one channel is used while the other
three are used for the VitDs. Thus, 8 injections from MMA and 36
injections of 25-OH-VitDs are completed in one hour.
Internal standard peak areas among blood serum specimens varied
greatly due to ion suppression by co-eluting interferences or by
sample matrix components that interfere with the butylation reaction.
The cause(s) of this variability is being investigated. However, the
calculated amounts of MMA concentrations in the QCs and specimens
All trademark
This informati
Start da
FIGURE 1
Desired q
Bup/Norbu
channels h
FIGURE 1