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High Resolution LC-MS for Screening and Quantitative Analysis of Antibiotics in Drinking Water Using an Orbitrap and Online Sample Preparation
Conclusion
This poster demonstrates:
Online pre-concentration and extraction for 1mL injections of antibiotics at the ppt
level.
The quantitation of HR/AM data using TraceFinder software from the Exactive
Plus Orbitrap instrument.
Spectral confirmation of MS
2
spectrum collected in the same data file as the
quantitation data using ExactFinder software.
Ability to quantitate and confirm samples in the same analytical run for antibiotics
in water samples.
Acknowledgements
We would like to thank Paul Yang from the Ontario Ministry for the Environment for
supplying the antibiotic standards for this analysis.
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ples, spectral
rformed using
fter acquisition. The
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ectra collected on
eters are
e 4. This
ard corresponding
rence spectrum.
reference
ntific LTQ Velos Pro
less background,
eless, the two
d molecular ion at
the possibility of
ems.
sses, LOQs and
)
% RSD at LOQ
8.90
14.30
9.90
13.50
4.20
7.30
4.80
4.90
3.45
6.30
6.60
3.60
13.10
FIGURE 4. Spectral comparision of the MS
2
spectrum of the antibiotic
trimethoprim obtained at a concentration of 80 pg/mL. The library reference
spectrum is the top spectrum, the lower spectrum is from the sample. The
comparison was performed with ExactFinder software.