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Forensic
Applications
LSD and Metabolites in Blood, Plasma, Serum and Urine
Using 200mg 3mL HyperSep Aminopropyl Extraction Column
(Part Number: 60108-425)
Sample Preparation
1mL of 100mM phosphate buffer (pH 6)
Add internal standards*
Add 1mL of whole blood, serum/plasma, urine
Add 2mL of 100mM phosphate buffer (pH 6)
Mix/vortex
Centrifuge
Condition HyperSep Aminopropyl Extraction Column
1 x 3mL CH
3
OH
1 x 3mL DI H
2
O
1 x 1mL 100mM phosphate buffer (pH 6)
Apply Sample
Load sample at 1 to 2mL/minute
Wash Column
1 x 3mL DI H
2
O
1 x 3mL 100mM acetic acid
1 x 3mL CH
3
OH
Dry column (5 minutes at >10"Hg)
Elute
1x 3mL ethyl acetate;
ACN:ammonia(78:20:2 v/v)
Collect eluate at 1 to 2mL/minute
Evaporate eluates to dryness under a gentle stream
of nitrogen
Analysis
Reconstitute sample in 50µL of CH
3
OH
Inject 5µL on to LC/MS
Mobile phase:
Time%
ACN%
0.1% Formic Acid
0
30
70
3.0
90
10
3.1
30
70
5.0
30
70
Flow rate: 0.5mL/minute
Column temperature: ambient
Detector: Thermo Scientific TSQ Triple Quadrupole
Compound
MRM Transition
LSD
324.2/223.1
Iso-LSD
324.2/281 (223.1)
Nor-LSD
310.2/209.1
OH-LSD
356.2/338.1
LSD-D3*
327.2/226.1
* Suggested internal standard: LSD-D3
Recovery: >90% (N=10)
LOD: 0.1ng/mL
Recommended HPLC Column
Part Number
Hypersil GOLD PFP 3µm, 100 x 2.1mm
25403-102130