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Lysergic Acid Diethylamide (LSD) in Serum, Plasma or
Whole Blood for GC or GC/MS Confirmations
Using 200mg 10mL HyperSep Verify-CX Extraction Column
(Part Number: 60108-742)
Prepare Sample
To 1mL of blood, plasma or serum, add 4mL of DI H
2
O
and internal standard*
Mix/vortex and let stand for 5 minutes
Centrifuge for 10 minutes at 2,000rpm and discard pellet
Add 2mL of 100mM phosphate buffer (pH 6.0)
Mix/vortex
Sample pH should be 6.0±0.5
Adjust pH accordingly with 100mM monobasic or dibasic
sodium phosphate
Condition Verify-CX Extraction Column
3mL of CH
3
OH then aspirate
3mL of DI H
2
O then aspirate
1mL of 100mM phosphate buffer (pH 6.0)
NOTE:
Aspirate at <3"Hg to prevent sorbent drying
Apply Sample
Load sample at 1 to 2mL/minute
Wash Column
3mL of DI H
2
O
3mL of 100mM acetic acid
3mL CH
3
OH
Dry column (5 minutes at >10"Hg)
Elute LSD
3mL of CH
2
Cl
2
/IPA/NH
4
OH (78:20:2)
Collect eluate at 1 to 2mL/min using minimal vacuum
NOTE:
Prepare elution solvent fresh daily; add IPA/NH
4
OH mix,
then add CH
2
Cl
2
(pH 11-12)
Dry Eluate
Evaporate to dryness at <40°C
Derivatize
Add 20µL ethyl acetate and 20µL BSTFA
(with 1% TMCS)***
Overlay with N
2
and cap
Mix/vortex
React for 30 minutes at 70°C
Remove from heat source to cool
NOTE:
Do not evaporate BSTFA solution
Quantitate
Inject 1 to 2µL onto gas chromatograph
For mass spectrometry, monitor the following ions:
Compound
Primary Ion** Secondary
Tertiary
D
3
-LSD-TMS*
298
296
271
LSD-TMS
395
293
268
* Suggested internal standard
** Quantitation ion
*** Part number TS-38831 (10 x 1mL ampules)
Recommended GC Columns
Part Number
TRACE TR-DoA5 Column, 15m x 0.25mm x 0.25µm
26AF130P
TRACE TR-DoA35 Column, 15m x 0.20mm x 0.33µm
26AC497P
100
Forensic
Applications