Page 204 - CRFTApplication Compendium_090613

6 Development of a Dilute-and-Shoot LC-MS/MS Method with 21 Opiates in Urine
Conclusion
A quick, automated multiplex LC-MS/MS method has been developed for monitoring
opiates in pain management forensic laboratories. This work demonstrates a
technically simple but robust quantitative method to simultaneously measure 21
opiates, metabolites and select glucuronides in human urine. This dilute-and-shoot
SRM LC-MS/MS methodology is suitable for use in high-throughput forensic
laboratories where sample volume is high with minimized amount of sample
preparation.
e, the matrix blank was screened to
as used as urine matrix to make
dilution of Cerilliant stock standards.
specimens as internal standards.
ng/ml, with linear response of three
rugs. Figure 4 shows the
tes.
deine, morphine-6-beta-D-
e. The lower left insert of the
rator 25 ng/ml.
at 25 ng/ml for all drugs. All
n 20% for the LLOQ and less than
retention times across two channels
for opiates (from immunoassay
dilute-and-shoot method with an
b. Variation of all positive opiate hits
30%. Further investigation of
ible explanation is that some of the
morphine sulphate, which is not
the opiates chromatogram layout of
FIGURE 5. Opiates chromatogram layout of pilot study urine sample A
For Forensic Use Only
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Fisher Scientific and its subsidiaries.
This information is not intended to encourage use of these products in any manners that might
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