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5 Thermo Scienti c Poster Note • PN ASMS13_M083_SSheng_E 07/13S
Conclusion
A quick, automated multiplex L
opiates in pain management f
technically simple but robust q
opiates, metabolites and selec
SRM LC-MS/MS methodology
laboratories where sample vol
preparation.
Prior to determining the calibration curve and range, the matrix blank was screened to
ensure there was no detectable opiates. Surine was used as urine matrix to make
calibration standards, which are made from serial dilution of Cerilliant stock standards.
The deuterated drug compounds were spiked into specimens as internal standards.
Calibration standards are in the range of 25-5000 ng/ml, with linear response of three
orders of magnitude (R2 > 0.99) for all analyzed drugs. Figure 4 shows the
representative calibration curves of selected analytes.
nides
e specificity of MRM measurements
et target ion ratios at all levels
of quantitative ion/qualifier ion at
FIGURE 4. Calibration curves of Morphine, Codeine, morphine-6-beta-D-
glucuronide and codeine-6-beta-D-glucuronide. The lower left insert of the
chromatogram display shows the lowest calibrator 25 ng/ml.
The limits of quantitation (LOQ) were determined at 25 ng/ml for all drugs. All
coefficients of variation (CV) (n=20) were less than 20% for the LLOQ and less than
10% for all other points of the curve. The CV% of retention times across two channels
is less than 1%.
Ten pilot study urine samples that tested positive for opiates (from immunoassay
screening) were used to test the correlation of the dilute-and-shoot method with an
acid hydrolysis method provided by a reference lab. Variation of all positive opiate hits
are within 20%, except for Morphine, which was <30%. Further investigation of
morphine discrepancy was undertaken. One possible explanation is that some of the
prescription morphine drug is actually made from morphine sulphate, which is not
included in the original analyte list. Figure 5 shows the opiates chromatogram layout of
one pilot study sample.
FIGURE 5. Opiates chromat
For Forensic Use
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Fisher Scientific and its subsidiaries.
This information is not intended to enc
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