Page 201 - CRFTApplication Compendium_090613

3 Thermo Scienti c Poster Note • PN ASMS13_M083_SSheng_E 07/13S
nts and narcotic analgesics,
management physicians need
ations as directed to monitor
agement forensic laboratories in
ple demand.
competitive immunoassays.
often lacks specificity, since the
, not at specific drug
C/MS, were used to identify or
C/MS requires extensive sample
tical run times.
piates testing in current pain
includes acid or enzyme
r to LC-MS/MS analysis. This
lyzed, but also suffers from
) and poor analyte recovery.
tant evidence that the parent
tes a simple dilute-and-shoot
mon glucuronides by liquid
S), without the limitation of
d drug coverage, fast turn-
lysis.
ater and 25 µl injected directly
tandard [IS}). A Thermo
ermo Scientific™ TSQ Quantum
nalysis in Selected Reaction
cronis™ C18, 3x100 mm 5 µm
The LC run time is 8 minutes
1 Opiates, metabolites and
d internal standards used for
k, TX). Surine was used as urine
samples were obtained from
olumn
tage quadrupole
Results and Discussions
A multiplex quantitative method suitable for use in the forensic environment has been
established. The 21 drugs included: fentanyl, norfentanyl, cis-tramadol, meperidine,
normeperidine, O-desmethyltramadol, methadone, EDDP, codeine, hydrocodone ,
oxycodone, oxymorphone, morphine, hydromorphone , noroxycodone,
norhydrocodone, Morphine-3-beta-D-glucuronide, morphine-6-beta-D-glucuronide,
codeine-6-beta-D-glucuronide, oxymorphone-3beta-D-glucuronide, and
hydromorphone-3-beta-D-glucuronide. Some of the drugs are isomers with the same
parent mass and fragmentation ions. The accurate quantitation of these drugs can
only be achieved by complete chromatographic separation.
We have optimized the LC separation by using several different column chemistries,
mobile phases and pH conditions. Figures 1 and 2 show the chromatogram layout of
16 opiates and 5 glucuronides by using the Thermo Scientific™ Syncronis™ C18,
3x100 mm, 5 µm columns.
Data Acquisition and Processing
Thermo Scientific™ Xcalibur™ and Thermo Scientific LCQuan™ software
Quality Control
Each analyte and internal standard were identified with ion ratio. All compounds have
to pass pre-set target ratios (qualifier ion/quantitation ion) at all calibration levels. The
ion ratios criteria is as follows:
>50% +/-20%
20-50% +/-25%
10-20% +/-30%
<10% +/-50%
Target ratio(%) Window(+/-%)
FIGURE 1. Chromatogram layout of 16 opiates.
FIGURE 2. Chromatogram layout
Stringent ion ratio criteria were use
in urine matrix. All compounds nee
(Figure 3).
FIGURE 3. Example of chromato
25 ng/ml level