Page 202 - CRFTApplication Compendium_090613

4 Development of a Dilute-and-Shoot LC-MS/MS Method with 21 Opiates in Urine
the forensic environment has been
tanyl, cis-tramadol, meperidine,
EDDP, codeine, hydrocodone ,
ne , noroxycodone,
morphine-6-beta-D-glucuronide,
-D-glucuronide, and
drugs are isomers with the same
quantitation of these drugs can
aration.
eral different column chemistries,
show the chromatogram layout of
Scientific™ Syncronis™ C18,
Prior to determining the calibrati
ensure there was no detectable
calibration standards, which are
The deuterated drug compound
Calibration standards are in the
orders of magnitude (R2 > 0.99)
representative calibration curves
fic LCQuan™ software
with ion ratio. All compounds have
n ion) at all calibration levels. The
)
FIGURE 2. Chromatogram layout of 5 glucuronides
Stringent ion ratio criteria were used to ensure the specificity of MRM measurements
in urine matrix. All compounds need to pass pre-set target ion ratios at all levels
(Figure 3).
FIGURE 3. Example of chromatogram overlay of quantitative ion/qualifier ion at
25 ng/ml level
FIGURE 4. Calibration curves
glucuronide and codeine-6-be
chromatogram display shows
The limits of quantitation (LOQ)
coefficients of variation (CV) (n=
10% for all other points of the cu
is less than 1%.
Ten pilot study urine samples th
screening) were used to test the
acid hydrolysis method provided
are within 20%, except for Morp
morphine discrepancy was unde
prescription morphine drug is ac
included in the original analyte li
one pilot study sample.