Introduction
3
Early History 3
Typical HILIC Stationary Phases and Applications 4
HILIC Retention Mechanism 6
HILIC: Advantages 8
HILIC: Limitations 10
HILIC Method Development
11
Know Your Analyte for HILIC Method Development 12
Stationary Phase Selection 14
The Mobile Phase in HILIC 19
The Effect of Column Temperature on HILIC Retention 27
Start up Guide 29
Troubleshooting
31
Column Care in HILIC
33
Stationary Phase Characterization Details
34
References
43
Table of Contents
