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Olanzapine in Whole Blood
Using 200mg 3mL HyperSep Cyano Extraction Column
(Part Number: 60108-747)
Prepare Sample
To 1mL of DI H
2
O add internal standard*
Add 1mL blood
Add 8mL of DI H
2
O
Mix/vortex and centrifuge as appropriate
Condition HyperSep Cyano Extraction Column
1 x 3mL CH
3
OH
1 x 3mL DI H
2
O
NOTE:
Aspirate at <3"Hg to prevent sorbent drying out
Apply Sample
Load sample at 1 to 2mL/minute
Wash Column
1 x 3mL 1% acetic acid (aq)
Dry column (5 minutes at >10"Hg)
Elute Olanzapine
2 x 3mL 1% acetic acid in CH
3
OH
Collect eluate at 1 to 2mL/minute
NOTE:
Prepare elution solvent daily
Evaporation
Evaporate eluates under a gentle stream of nitrogen <40°C
Reconstitute
Reconstitute sample in 100µL 0.1% trifluoroacetic acid (aq)
Inject 50µL on to LC-UV (260nm)
* Suggested internal standard: Prazepam
Recommended HPLC Column
Part Number
Hypersil GOLD 3µm, 150 x 4.6mm
25003-154630
Oleic Acid and its Metabolites in Blood Plasma
Using 200mg 3mL HyperSep Retain-AX Extraction Column
(Part Number: 60107-401)
Prepare Sample
Add 3% phosphoric acid to sample to 500µL of sample
Condition HyperSep Retain-AX Extraction Column
1 x 1mL CH
3
OH
1 x 1mL H
2
O
Apply Sample
Load 0.5mL of sample
Wash Column
1 x 1mL 1%CH
4
O
3
S
1mL CH
3
OH
Elute Compound
1 x 1mL ACN(1% formic acid)
Quantitation
Inject sample on to LC/MS/MS
Mobile phase:
ACN:3mmol/Lammonium acetate (85:15)
Compound
MRM Transition
Oleic acid
281.2/281.2
Oleic acid metabolite
315.2/315.2
Internal label C17
269.2/269.2 (internal label C17)
Recommended HPLC Column
Part Number
Hypersil GOLD 5µm, 150 x 4.6mm
25005-154630
Pharmaceutical/Biotech
Applications