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Lovastatin in Water
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
Sample 1: 2ppm lovastatin in 10% acetonitrile aqueous
solution
Sample 2: 0.2ppm lovastatin in 1% acetonitrile aqueous
solution
Condition Retain PEP Extraction Column
2mL of methanol
2mL of DI H
2
O
Apply Sample
Load 10mL samples at 1 to 2mL/minute
Wash Column
1mL of DI H
2
O
Elute Lovastatin
4mL of acetonitrile
Dry Eluate and Reconstitute
Evaporate to full dryness under a stream of nitrogen
Reconstitute the sample to 1mL with
methanol:1% formic acid solution (85:15, v/v)
Analysis
Mobile phase: methanol:1% formic acid solution (85:15, v/v)
Flow: 1mL/minute
Temperature: 30°C
Detection: UV 230nm
Recommended HPLC Column
Part Number
Hypersil GOLD aQ 5µm, 150 x 4.6mm
25305-154630
Ketotifen Fumarate in Solution
Using 60mg 3mL HyperSep Retain PEP Extraction Column
(Part Number: 60107-203)
Sample Preparation
Dilute 50mg of ketotifen fumarate in 50mL of 0.5%
ammonia aqueous solution
Condition Retain PEP Extraction Column
2mL of methanol
2mL of DI H
2
O
Apply Sample
Load 2mL samples
Wash Column
2mL of DI H
2
O
Elute Ketotifen Fumarate
2mL of methanol
Dry Eluate and Reconstitute
Evaporate to full dryness at room temperature under a
stream of nitrogen
Reconstitute sample in suitable solvent for analysis
Pharmaceutical/Biotech
Applications
Methylmalonic Acid from Serum or Plasma for GC/MS Analysis
Using 500mg 6mL HyperSep SAX Extraction Column
(Part Number: 60108-434)
Prepare Sample
Add 100µL of internal standard D3-MMA and 1mL of
acetonitrile to 1mL of plasma or serum
Mix/vortex for 20 seconds
Centrifuge for 5 min at 2,000rpm
Condition Extraction Column
1 x 3mL CH
3
OH
1 x 3mL DI H
2
O
Apply Sample
Decant supernatant onto SPE column
Wash Column
1 x 10mL of DI H
2
O
Dry with vacuum for 3 min
1 x 5mL of CH
3
OH
Dry with vacuum for 3 min
1 x 2mL of MTBE*
Dry with vacuum for 3 min
Elute Methylmalonic Acid
1 x 5mL of 3% formic acid in MTBE, collect at 1 to
2mL/min
Dry Eluate
Dry under a stream of nitrogen at <35°C
Derivatize
Reconstitute with 25µL of MSTFA + 1% TMCS and
25µL ethyl acetate
Heat for 20 min at 60°C
Quantitate
Inject 1 to 2µL onto GC/MS
Recommended GC Column
Part Number
TraceGOLD TG-5MS 30m x 0.25m x 0.25µm
26098-1420